| Title | Effect of promoter-upstream sequence on σ38-dependent stationary phase gene transcription |
|---|---|
| Author | Hyung-Ju Lim, Kwangsoo Kim, Minsang Shin, Jae-Ho Jeong, Phil Youl Ryu, and Hyon E. Choy* |
| Address | Department of Microbiology, Chonnam National University Medical School, Kwangju 501-746, Republic of Korea |
| Bibliography | Journal of Microbiology, 53(4),250-255, 2015, |
| DOI | 10.1007/s12275-015-4681-8 |
| Key Words | E. coli, promoter specificity, RNA polymerase, rpoS, σ38 |
| Abstract | σ38 in Escherichia coli is required for expression of a subset of stationary phase genes. However, the promoter elements for σ38-dependent genes are virtually indistinguishable from that for σ70-dependent house-keeping genes. hdeABp is a σ38-dependent promoter and LEE5p is a σ70-dependent promoter, but both are repressed by H-NS, a bacterial histone- like protein, which acts at promoter upstream sequence. We swapped the promoter upstream sequences of the two promoters and found that the σ dependency was switched. This was further verified using lacUV5 core promoter. The results suggested that the determinant for σ38-dependent promoter lies in the promoter upstream sequence. |