Title |
Carbohydrate-binding specificities of potential probiotic Lactobacillus strains in porcine jejunal (IPEC-J2) cells and porcine mucin |
Author |
Valerie Diane Valeriano, Bernadette B. Bagon, Marilen P. Balolong, and Dae-Kyung Kang* |
Address |
Department of Animal Resource Science, Dankook University, Cheonan 31116, Republic of Korea |
Bibliography |
Journal of Microbiology, 54(7),510-519, 2016,
|
DOI |
10.1007/s12275-016-6168-7
|
Key Words |
porcine mucin, IPEC-J2, adhesion, Lactobacillus mucosae, Lactobacillus johnsonii, carbohydrate-binding specificity |
Abstract |
Bacterial lectins are carbohydrate-binding adhesins that recognize
glycoreceptors in the gut mucus and epithelium of
hosts. In this study, the contribution of lectin-like activities
to adhesion of Lactobacillus mucosae LM1 and Lactobacillus
johnsonii PF01, which were isolated from swine intestine,
were compared to those of the commercial probiotic Lactobacillus
rhamnosus GG. Both LM1 and PF01 strains have
been reported to have good adhesion ability to crude intestinal
mucus of pigs. To confirm this, we quantified their adhesion
to porcine gastric mucin and intestinal porcine enterocytes
isolated from the jejunum of piglets (IPEC-J2). In addition,
we examined their carbohydrate-binding specificities by suspending
bacterial cells in carbohydrate solutions prior to adhesion
assays. We found that the selected carbohydrates affected
the adherences of LM1 to IPEC-J2 cells and of LGG to
mucin. In addition, compared to adhesion to IPEC-J2 cells,
adhesion to mucin by both LM1 and LGG was characterized
by enhanced specific recognition of glycoreceptor components
such as galactose, mannose, and N-acetylglucosamine.
Hydrophobic interactions might make a greater contribution
to adhesion of PF01. A similar adhesin profile between a probiotic
and a pathogen, suggest a correlation between shared
pathogen–probiotic glycoreceptor recognition and the ability
to exclude enteropathogens such as Escherichia coli K88 and
Salmonella Typhimurium KCCM 40253. These findings extend
our understanding of the mechanisms of the intestinal
adhesion and pathogen-inhibition abilities of probiotic Lactobacillus
strains. |