Title |
Lactobacillus curvatus WiKim38 isolated from kimchi induces IL-10 production in dendritic cells and alleviates DSS-induced colitis in mice |
Author |
Sung-Gang Jo1, Eui-Jeong Noh2, Jun-Young Lee1, Green Kim1,3, Joo-Hee Choi1, Mo-Eun Lee4, Jung-Hee Song4, Ji-Yoon Chang4*, and Jong-Hwan Park1* |
Address |
1Laboratory Animal Medicine and BK21 PLUS Project Team, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Republic of Korea, 2Department of Biochemistry, College of Medicine, Konyang University, Daejeon 35365, Republic of Korea, 3National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk 28116, Republic of Korea, 4Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea |
Bibliography |
Journal of Microbiology, 54(7),503-509, 2016,
|
DOI |
10.1007/s12275-016-6160-2
|
Key Words |
Lactobacillus curvatus, kimchi, interleukin-10, dendritic cells, colitis |
Abstract |
Probiotics such as lactobacilli and bifidobacteria have healthpromoting
effects by immune modulation. In the present
study, we examined the immunomodulatory properties of
Lactobacillus curvatus WiKim38, which was newly isolated
from baechu (Chinese cabbage) kimchi. The ability of L.
curvatus WiKim38 to induce cytokine production in bone
marrow-derived dendritic cells (BMDCs) was determined by
enzyme-linked immunosorbent assay. To evaluate the molecular
mechanisms underlying L. curvatus Wikim38-mediated
IL-10 production, Western blot analyses and inhibitor
assays were performed. Moreover, the in vivo anti-inflammatory
effects of L. curvatus WiKim38 were examined in a dextran
sodium sulfate (DSS)-induced colitis mouse model. L.
curvatus WiKim38 induced significantly higher levels of IL-
10 in BMDCs compared with that induced by LPS. NF-κB
and ERK were activated by L. curvatus WiKim38, and an
inhibitor assay revealed that these pathways were required
for L. curvatus WiKim38-induced production of IL-10 in
BMDCs. An in vivo experiment showed that oral administration
of L. curvatus WiKim38 increased the survival rate
of mice with DSS-induced colitis and improved clinical signs
and histopathological severity in colon tissues. Taken together,
these results indicate that L. curvatus Wikim38 may
have health-promoting effects via immune modulation, and
may thus be applicable for therapy of various inflammatory
diseases. |