Title Lactobacillus curvatus WiKim38 isolated from kimchi induces IL-10 production in dendritic cells and alleviates DSS-induced colitis in mice
Author Sung-Gang Jo1, Eui-Jeong Noh2, Jun-Young Lee1, Green Kim1,3, Joo-Hee Choi1, Mo-Eun Lee4, Jung-Hee Song4, Ji-Yoon Chang4*, and Jong-Hwan Park1*
Address 1Laboratory Animal Medicine and BK21 PLUS Project Team, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Republic of Korea, 2Department of Biochemistry, College of Medicine, Konyang University, Daejeon 35365, Republic of Korea, 3National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk 28116, Republic of Korea, 4Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea
Bibliography Journal of Microbiology, 54(7),503-509, 2016,
DOI 10.1007/s12275-016-6160-2
Key Words Lactobacillus curvatus, kimchi, interleukin-10, dendritic cells, colitis
Abstract Probiotics such as lactobacilli and bifidobacteria have healthpromoting effects by immune modulation. In the present study, we examined the immunomodulatory properties of Lactobacillus curvatus WiKim38, which was newly isolated from baechu (Chinese cabbage) kimchi. The ability of L. curvatus WiKim38 to induce cytokine production in bone marrow-derived dendritic cells (BMDCs) was determined by enzyme-linked immunosorbent assay. To evaluate the molecular mechanisms underlying L. curvatus Wikim38-mediated IL-10 production, Western blot analyses and inhibitor assays were performed. Moreover, the in vivo anti-inflammatory effects of L. curvatus WiKim38 were examined in a dextran sodium sulfate (DSS)-induced colitis mouse model. L. curvatus WiKim38 induced significantly higher levels of IL- 10 in BMDCs compared with that induced by LPS. NF-κB and ERK were activated by L. curvatus WiKim38, and an inhibitor assay revealed that these pathways were required for L. curvatus WiKim38-induced production of IL-10 in BMDCs. An in vivo experiment showed that oral administration of L. curvatus WiKim38 increased the survival rate of mice with DSS-induced colitis and improved clinical signs and histopathological severity in colon tissues. Taken together, these results indicate that L. curvatus Wikim38 may have health-promoting effects via immune modulation, and may thus be applicable for therapy of various inflammatory diseases.