Title |
Epidemiology and resistance features of Acinetobacter baumannii isolates from the ward environment and patients in the burn ICU of a Chinese hospital |
Author |
Yali Gong1,2, Xiaodong Shen3, Guangtao Huang2, Cheng Zhang2, Xiaoqiang Luo2, Supeng Yin2, Jing Wang1, Fuquan Hu1, Yizhi Peng2*, and Ming Li1* |
Address |
1Department of Microbiology, Third Military Medical University, Chongqing 400038, P. R. China, 2Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, P. R. China, 3Department of Biochemistry and Molecular Biology, Third Military Medical University, Chongqing 400038, P. R. China |
Bibliography |
Journal of Microbiology, 54(8),551-558, 2016,
|
DOI |
10.1007/s12275-016-6146-0
|
Key Words |
Acinetobacter baumannii, nosocomial infection,
burn ICU, epidemiology, resistance |
Abstract |
Acinetobacter baumannii is an important opportunistic pathogen
that causes severe nosocomial infections, especially
in intensive care units (ICUs). Over the past decades, an everincreasing
number of hospital outbreaks caused by A. baumannii
have been reported worldwide. However, little attention
has been directed toward the relationship between A. baumannii
isolates from the ward environment and patients in
the burn ICU. In this study, 88 A. baumannii isolates (26 from
the ward environment and 62 from patients) were collected
from the burn ICU of the Southwest Hospital in Chongqing,
China, from July through December 2013. Antimicrobial susceptibility
testing results showed that drug resistance was more
severe in isolates from patients than from the ward environment,
with all of the patient isolates being fully resistant to
10 out of 19 antimicrobials tested. Isolations from both the
ward environment and patients possessed the β-lactamase
genes blaOXA-51, blaOXA-23, blaAmpC, blaVIM, and blaPER. Using
pulsed-field gel electrophoresis (PFGE) and multi-locus sequence
typing (MLST), these isolates could be clustered into
4 major PFGE types and 4 main sequence types (ST368, ST369,
ST195, and ST191) among which, ST368 was the dominant
genotype. Epidemiologic and molecular typing data also revealed
that a small-scale outbreak of A. baumannii infection
was underway in the burn ICU of our hospital during the
sampling period. These results suggest that dissemination
of β-lactamase genes in the burn ICU might be closely associated
with the high-level resistance of A. baumannii, and
the ICU environment places these patients at a high risk for
nosocomial infection. Cross-contamination should be an
important concern in clinical activities to reduce hospital acquired infections caused by A. baumannii. |