| Title | Deinococcus seoulensis sp. nov., a bacterium isolated from sediment at Han River in Seoul, Republic of Korea |
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| Author | Jae-Jin Lee1, Yeon-Hee Lee1, Su-Jin Park1, Sangyong Lim2, Sun-Wook Jeong2, Seung-Yeol Lee1, Young-Je Cho3, Myung Kyum Kim4, and Hee-Young Jung1,5* |
| Address | 1School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea, 2Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea, 3School of Food Science and Biotechnology/Food and Bio-Industry Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea, 4Department of Bio and Environmental Technology, Seoul Women’s University, Seoul 01797, Republic of Korea, 5Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Republic of Korea |
| Bibliography | Journal of Microbiology, 54(8),537-542, 2016, |
| DOI | 10.1007/s12275-016-6253-y |
| Key Words | Deinococcus, polyphasic taxonomy, gamma-radiation resistance |
| Abstract | Strain 16F1ET was isolated from a 3-kGy-irradiated sediment sample collected at Han River in Seoul, Republic of Korea. Cells of this strain were observed to be Gram-positive, pililike structure, and short rod shape, and colonies were red in color. The strain showed the highest degree of 16S rRNA gene sequence similarity to Deinococcus aquaticus PB314T (98.8%), Deinococcus depolymerans TDMA-24T (98.1%), Deinococcus caeni Ho-08T (98.0%), and Deinococcus grandis DSM 3963T (97.0%). 16S rRNA gene sequence analysis identified this strain as a member of the genus Deinococcus (Family: Deinococcaceae). The genomic DNA G+C content of strain 16F1ET was 66.9 mol%. The low levels of DNA-DNA hybridization (< 56.2%) with the species mentioned above identified strain 16F1ET as a novel Deinococcus species. Its oxidase and catalase activities as well as the production of acid from glucose were positive. Growth of the strain was observed at 10–37°C (optimum: 20–30°C) and pH 4–10 (optimum: pH 7–8). The cells tolerated less than 5% NaCl and had low resistance to gamma radiation (D10 < 4 kGy). Strain 16F1ET possessed the following chemotaxonomic characteristics: C16:0, C15:1 ω6c, and C16:1 ω7c as the major fatty acids; phosphoglycolipid as the predominant polar lipid; and menaquinone-8 as the predominant respiratory isoprenoid quinone. Based on the polyphasic evidence, as well as the phylogenetic, genotypic, phenotypic, and chemotaxonomic characterization results, strain 16F1ET (=KCTC 33793T =JCM 31404T) is proposed to represent the type strain of a novel species, Deinococcus seoulensis sp. nov. |