Title |
MINIREVIEW] On the study of microbial transcriptomes using second- and third-generation sequencing technologies |
Author |
Sang Chul Choi* |
Address |
Department of Biology, Sungshin Women's University, Seoul 01133, Republic of Korea |
Bibliography |
Journal of Microbiology, 54(8),527-536, 2016,
|
DOI |
10.1007/s12275-016-6233-2
|
Key Words |
library preparation, bioinformatics, quality control,
RNA-seq, differential expression, functional enrichment,
Oxford Nanopore MinION |
Abstract |
Second-generation sequencing technologies transformed the
study of microbial transcriptomes. They helped reveal the
transcription start sites and antisense transcripts of microbial
species, improving the microbial genome annotation.
Quantification of genome-wide gene expression levels allowed
for functional studies of microbial research. Ever-evolving
sequencing technologies are reshaping approaches to studying
microbial transcriptomes. Recently, Oxford Nanopore
Technologies delivered a sequencing platform called MinION,
a third-generation sequencing technology, to the research
community. We expect it to be the next sequencing technology
that enables breakthroughs in life science fields. The studies
of microbial transcriptomes will be no exception. In this paper,
we review microbial transcriptomics studies using second-
generation sequencing technology. We also discuss the
prospect of microbial transcriptomics studies with thirdgeneration
sequencing. |