| Title | MDA7/IL-24 is an anti-viral factor that inhibits influenza virus replication |
|---|---|
| Author | Rak-Kyun Seong1, Young-Ki Choi2, and Ok Sarah Shin1* |
| Address | 1Brain Korea 21 Plus for Biomedical Science, College of Medicine, Korea University, Seoul 08308, Republic of Korea, 2College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju 28644, Republic of Korea |
| Bibliography | Journal of Microbiology, 54(10),695-700, 2016, |
| DOI | 10.1007/s12275-016-6383-2 |
| Key Words | IL-24, influenza, antiviral activities |
| Abstract | Melanoma differentiation associated gene-7 (mda-7)/interleukin- 24 (IL-24) is a secreted cytokine, which plays an essential role in tumor suppression. Although its role as a multifunctional protein affecting broad types of cancers is well described, functions of IL-24 in host defense against virus infection are yet to be determined. In this study, we explored the anti-viral effect of recombinant IL-24 treatment during influenza infection. Infection of human lung adenocarcinoma cells (A549) with the influenza A virus up-regulated IL-24 mRNA and protein expression in a time-dependent manner. Pre-treatment of A549 cells with recombinant IL-24 protein effectively suppressed viral plaque formation. Furthermore, IL-24 treatment of A549 cells reduced viral non-structural protein 1 (NS1) synthesis, whereas IL-24 knockdown resulted in increased viral replication. Interestingly, IL-24 treatment following influenza A virus infection led to up-regulation of interferon (IFN)-induced antiviral signaling. Taken together, our results suggest that IL-24 exerts a potent suppressive effect on influenza viral replication and can be used in the treatment of influenza infection. |