Title |
Arcobacter acticola sp. nov., isolated from seawater on the East Sea in South Korea |
Author |
Sooyeon Park1, Yong-Taek Jung1,2, Sona Kim1, and Jung-Hoon Yoon1* |
Address |
1Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon 16419, Republic of Korea, 2University of Science and Technology (UST), Daejeon 34113, Republic of Korea |
Bibliography |
Journal of Microbiology, 54(10),655-659, 2016,
|
DOI |
10.1007/s12275-016-6268-4
|
Key Words |
Arcobacter acticola, East Sea, novel species, polyphasic
taxonomy, seawater |
Abstract |
A Gram-stain-negative, facultative aerobic, non-flagellated,
and rod-shaped bacterium, designated AR-13T, was isolated
from a seawater on the East Sea in South Korea, and subjected
to a polyphasic taxonomic study. Strain AR-13T grew
optimally at 30°C, at pH 7.0–8.0 and in the presence of
0–0.5% (w/v) NaCl. The phylogenetic trees based on 16S
rRNA gene sequences showed that strain AR-13T fell within
the clade comprising the type strains of Arcobacter species,
clustering coherently with the type strain of Arcobacter venerupis.
Strain AR-13T exhibited 16S rRNA gene sequence
similarity values of 98.1% to the type strain of A. venerupis
and of 93.2–96.9% to the type strains of the other Arcobacter
species. Strain AR-13T contained MK-6 as the only menaquinone
and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c),
C16:0, C18:1 ω7c, and summed feature 2 (iso-C16:1 I and/or
C14:0 3-OH) as the major fatty acids. The major polar lipids
detected in strain AR-13T were phosphatidylethanolamine,
phosphatidylglycerol, and one unidentified aminophospholipid.
The DNA G+C content was 28.3 mol% and its mean
DNA-DNA relatedness value with the type strain of A. venerupis
was 21%. Differential phenotypic properties, together
with its phylogenetic and genetic distinctiveness, revealed
that strain AR-13T is separated from recognized Arcobacter
species. On the basis of the data presented, strain AR-13T is
considered to represent a novel species of the genus Arcobacter,
for which the name Arcobacter acticola sp. nov. is
proposed. The type strain is AR-13T (=KCTC 52212T =NBRC
112272T). |