Title |
Reovirus safety study for proliferation and differentiation of human adipose-derived mesenchymal stem cells |
Author |
Jeong-Soo Park1 and Manbok Kim2* |
Address |
1Department of Biochemistry, Dankook University College of Medicine, Cheonan 31116, Republic of Korea, 2Department of Medical Science, Dankook University College of Medicine, Cheonan 31116, Republic of Korea |
Bibliography |
Journal of Microbiology, 55(1),75-79, 2017,
|
DOI |
10.1007/s12275-017-6542-0
|
Key Words |
oncolytic virus, reovirus, safety, human adiposederived
mesenchymal stem cells |
Abstract |
Naturally occurring reoviruses are live replication-proficient
viruses specifically infecting human cancer cells while sparing
the normal counterparts. Stem cells can be highly susceptible
to viral infection due to their innate high proliferation potential
and other active signaling pathways of cells that might be
involved in viral tropism. In the previous study, we showed
that reoviruses could adversely affect murine embryonic stem
cells’ integrity in vitro and in vivo. Oncolytic viruses, delivered
systemically face many hurdles that also impede their
localization and infection of, metastatic tumors, due to a variety
of immune and physical barriers. To overcome such hurdles
to systemic delivery, several studies supported the idea
that certain types of cells, including mesenchymal stem cells,
might play a role as cell carriers for oncolytic viruses. Thus, it
would be interesting to examine whether human adult stem
cells such as human adipose-derived mesenchymal stem cells
could be saved by the reoviral challenge. In this study, we report
that biological activities such as proliferation and multipotency
of human adipose-derived stem cells are not affected
by wild-type reovirus challenge as evidenced by survival, osteogenic
and adipogenic differentiation potential assays following
treatment with reoviruses. Therefore, unlike murine
embryonic stem cells, our study strongly suggests that human
adipose-derived adult stem cells could be spared in vivo during
wild-type reoviral anti-cancer therapeutics in a clinical setting.
Furthermore, the results support the possible clinical use
of human adipose-derived stem cells as an effective cell carrier
of oncolytic reovirus to maximize their tumor tropism
and anti-tumor activity. |