Title |
Performance of nested multiplex PCR assay targeting MTP40 and IS6110 gene sequences for the diagnosis of tubercular lymphadenitis |
Author |
Pallavi Sinha1, Pradyot Prakash1, Shashikant C.U. Patne2, Shampa Anupurba1, Sweety Gupta1, and G. N. Srivastava3 |
Address |
1Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India, 2Department of Pathology, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India, 3Department of Respiratory Diseases, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India |
Bibliography |
Journal of Microbiology, 55(1),63-67, 2017,
|
DOI |
10.1007/s12275-017-6127-y
|
Key Words |
fine needle aspiration, Mycobacterium tuberculosis,
nested multiplex PCR, Ziehl-Neelsen staining |
Abstract |
The conventional methods for diagnosis of tubercular lymphadenitis
(TBLN) such as - fine needle aspiration cytology,
Ziehl-Neelsen staining and culture have limitations of low
sensitivity and/or specificity. So, it becomes essential to develop
a rapid, sensitive, and specific method for an early diagnosis
of TBLN. Therefore, the present study was conducted
to evaluate nested multiplex polymerase chain reaction
(nMPCR) targeting MTP40 and IS6110 gene sequences
of Mycobacterium tuberculosis and Mycobacterium
tuberculosis complex, respectively in 48 successive patients
of TBLN and 20 random patients with non-tubercular lymph
node lesions. Out of the 48 cases of TBLN, 14 (29.2%) were
found to be positive by Ziehl-Neelsen staining, 15 (31.2%)
were positive by culture and 43 (89.6%) cases were positive
after first round of PCR while 48 (100%) cases were positive
by nMPCR assay. The sensitivity and specificity of nMPCR
was found to be 100% for the diagnosis of TBLN. The results
thus obtained indicate that nMPCR assay is a highly sensitive
and specific tool for the diagnosis of TBLN. |