Title |
RraAS1 inhibits the ribonucleolytic activity of RNase ES by interacting with its catalytic domain in Streptomyces coelicolor |
Author |
Sojin Seo, Daeyoung Kim, Wooseok Song, Jihune Heo, Minju Joo, Yeri Lim, Ji-Hyun Yeom*, and Kangseok Lee* |
Address |
Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea |
Bibliography |
Journal of Microbiology, 55(1),37-43, 2017,
|
DOI |
10.1007/s12275-017-6518-0
|
Key Words |
Streptomyces coelicolor, RNase E, RNase ES, RraA, RraAS1 |
Abstract |
RraA is a protein inhibitor of RNase E, which degrades and
processes numerous RNAs in Escherichia coli. Streptomyces
coelicolor also contains homologs of RNase E and RraA,
RNase ES and RraAS1/RraAS2, respectively. Here, we report
that, unlike other RraA homologs, RraAS1 directly interacts
with the catalytic domain of RNase ES to exert its inhibitory
effect. We further show that rraAS1 gene deletion in S. coelicolor
results in a higher growth rate and increased production
of actinorhodin and undecylprodigiosin, compared with
the wild-type strain, suggesting that RraAS1-mediated regulation
of RNase ES activity contributes to modulating the
cellular physiology of S. coelicolor. |