Title |
Cyclobacterium sediminis sp. nov. isolated from a sea cucumber aquaculture farm and emended description of the genus Cyclobacterium |
Author |
Seyeon Shin1 and Hyung-Yeel Kahng1,2* |
Address |
1Department of Environmental Education, Sunchon National University, Suncheon 57922, Republic of Korea, 2Research Institute for Sanitation and Environment of Coastal Areas, Sunchon National University, Suncheon 57922, Republic of Korea |
Bibliography |
Journal of Microbiology, 55(2),90-95, 2017,
|
DOI |
10.1007/s12275-017-6484-6
|
Key Words |
polyphasic study, sea cucumber, aquaculture farm
sediment, Cyclobacterium sediminis sp. nov. |
Abstract |
An aerobic, Gram-negative bacterium, designated strain
SD70T, was isolated from sea cucumber aquaculture farm
sediment in Taean, Korea, and its taxonomic status was established
by undertaking a polyphasic study. Cells of strain
SD70T were non-motile, catalase-, and oxidase-positive, nonspore-
forming, and horseshoe-shaped. Optimal growth was
observed under 25–30°C, pH 7.0–8.0, and 3.0–5.0% (w/v)
NaCl conditions. Phylogenetic analyses based on 16S rRNA
gene sequences revealed that strain SD70T fell within an
evolutionary group comprising species of the genus Cyclobacterium.
Strain SD70T shared 92.1–98.5% 16S rRNA sequence
similarity values with the type strains of species of the
genus Cyclobacterium. Relatively low levels of DNA-DNA
relatedness were found between strain SD70T and C. marinum
DSM 745T (40.2%) and C. amurskyense KMM 6143T
(15.8%). The predominant cellular fatty acids were iso-C15:0
(32.1%), and anteiso-C15:0 (9.1%). Menaquinone MK-7 was
the only respiratory quinone. The G+C content of the genomic
DNA was 36 mol%. The polar lipids were phosphatidylcholine,
phosphatidylethanolamine, phosphatidylglycerol,
and seven unidentified lipids. On the basis of phenotypic and
genotypic data, strain SD70T represents a novel species of the
genus Cyclobacterium, for which the name Cyclobacterium
sediminis sp. nov. is proposed. An emended description of
the genus Cyclobacterium is also provided. |