| Title | Promising cellulolytic fungi isolates for rice straw degradation |
|---|---|
| Author | Diana Catalina Pedraza-Zapata1, Andrea Melissa Sánchez-Garibello1,2, Balkys Quevedo-Hidalgo3, Nubia Moreno-Sarmiento1, and Ivonne Gutiérrez-Rojas2* |
| Address | 1Grupo de Bioprocesos y Bioprospección, Instituto de Biotecnología, Universidad Nacional de Colombia, Sede Bogotá, 111321, Bogotá, D.C., Colombia, 2Laboratorio de Biotecnología Aplicada, Grupo de Biotecnología Ambiental e Industrial (GBAI), Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, 110231, Bogotá, D.C., Colombia, 3Grupo de Investigación en Agricultura Biológica. Facultad de Ciencias, Pontificia Universidad Javeriana, 110231, Bogotá, D.C., Colombia |
| Bibliography | Journal of Microbiology, 55(9),711–719, 2017, |
| DOI | 10.1007/s12275-017-6282-1 |
| Key Words | rice straw, biodegradation, Pleurotus ostreatus, Penicillium sp., ligninolytic enzymes, cellulolytic enzymes |
| Abstract | The objective of this study was to evaluate the potential of eight fungal isolates obtained from soils in rice crops for straw degradation in situ. From the initial eight isolates, Pleurotus ostreatus T1.1 and Penicillium sp. HC1 were selected for further characterization based on qualitative cellulolytic enzyme production and capacity to use rice straw as a sole carbon source. Subsequently, cellulolytic, xylanolytic, and lignolytic (Pleurotus ostreatus) activity on carboxymethyl cellulose, oat xylan, and rice straw with different nitrogen sources was evaluated. From the results obtained it was concluded both isolates are capable to produce enzymes necessary for rice straw degradation. However, their production is dependent upon carbon and nitrogen source. Last, it was established that Pleurotus ostreatus T1.1 and Penicillium sp. HC1 capability to colonize and mineralize rice straw, in mono-and co-culture, without affecting nitrogen soil content. |