Title |
A novel methanotroph in the genus Methylomonas that contains a distinct clade of soluble methane monooxygenase |
Author |
Ngoc-Loi Nguyen1, Woon-Jong Yu1, Hye-Young Yang1, Jong-Geol Kim1, Man-Young Jung2, Soo-Je Park3, Seong-Woon Roh4, and Sung-Keun Rhee1* |
Address |
1Department of Microbiology, Chungbuk National University, Cheongju 28644, Republic of Korea, 2Department of Microbiology and Ecosystem Science, Division of Microbial Ecology, University of Vienna, 1090 Vienna, Austria, 3Department of Biology, Jeju National University, Jeju 63243, Republic of Korea, 4World Institute of Kimchi, Gwangju 61755, Republic of Korea |
Bibliography |
Journal of Microbiology, 55(10),775–782, 2017,
|
DOI |
10.1007/s12275-017-7317-3
|
Key Words |
methanotroph, Methylomonas, soluble methane
monooxygenase, phylogeny |
Abstract |
Aerobic methane oxidation is a key process in the global carbon
cycle that acts as a major sink of methane. In this study,
we describe a novel methanotroph designated EMGL16-1
that was isolated from a freshwater lake using the floating
filter culture technique. Based on a phylogenetic analysis of
16S rRNA gene sequences, the isolate was found to be closely
related to the genus Methylomonas in the family Methylococcaceae
of the class Gammaproteobacteria with 94.2–97.4%
16S rRNA gene similarity to Methylomonas type strains. Comparison
of chemotaxonomic and physiological properties
further suggested that strain EMGL16-1 was taxonomically
distinct from other species in the genus Methylomonas. The
isolate was versatile in utilizing nitrogen sources such as molecular
nitrogen, nitrate, nitrite, urea, and ammonium. The
genes coding for subunit of the particulate form methane
monooxygenase (pmoA), soluble methane monooxygenase
(mmoX), and methanol dehydrogenase (mxaF) were detected
in strain EMGL16-1. Phylogenetic analysis of mmoX indicated
that mmoX of strain EMGL16-1 is distinct from those
of other strains in the genus Methylomonas. This isolate probably
represents a novel species in the genus. Our study provides
new insights into the diversity of species in the genus
Methylomonas and their environmental adaptations. |