| Title | Rhodoferax koreense sp. nov, an obligately aerobic bacterium within the family Comamonadaceae, and emended description of the genus Rhodoferax |
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| Author | Mohamed El-Agamy Farh1, Yeon-Ju Kim2*, Priyanka Singh2, Sun Young Jung2, Jong-Pyo Kang2, and Deok-Chun Yang1,2* |
| Address | 1Graduate School of Biotechnology and Ginseng Bank, Kyung Hee University, Yongin 17104, Republic of Korea, 2Department of Oriental Medicinal Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea |
| Bibliography | Journal of Microbiology, 55(10),767–774, 2017, |
| DOI | 10.1007/s12275-017-7033-z |
| Key Words | taxonomy, Proteobacteria, Rhodoferax koreense sp. nov, sludge |
| Abstract | Gram-staining-negative, uniflagellated, rod-shaped, designated as DCY110T, was isolated from sludge located in Gangwon province, Republic of Korea. The phylogenetic tree of 16S rRNA gene sequence showed that the strain DCY110T belonged to the genus Rhodoferax with a close similarity to Rhodoferax saidenbachensis DSM 22694T (97.7%), Rhodoferax antarcticus DSM 24876T (97.5%), Rhodoferax ferrireducens DSM 15236T (97.3%), and Rhodoferax fermentans JCM 7819T (96.7%). The predominant isoprenoid quinine was ubiquinone (Q-8). DNA G + C content was 62.8 mol%. The major polar lipids were phosphatidylethanolamine and two unidentified phospholipids. The major fatty acids (> 10%) were C12:0, C16:0, summed feature 3 (which comprised C16:1 ω7c and/or C16:1 ω6c). The DNA-DNA relatedness values between the strain DCY110T and the closely related relatives used in this study were lower than 70%. Based on the following polyphasic analysis, the strain DCY110T is considered as a novel species of the genus Rhodoferax, for which the name Rhodoferax koreense sp. nov. is proposed. The type strain is DCY- 110T (= KCTC 52288T = JCM 31441T). |