Title |
Lysobacter spongiae sp. nov., isolated from spongin |
Author |
Heejae Choi1, Wan-Taek Im2*, and Jin-Sook Park1* |
Address |
1Department of Biological Sciences and Biotechnology, Hannam University, Daejeon 34430, Republic of Korea, 2Department of Biotechnology, Hankyong National University, Anseong 17579, Republic of Korea |
Bibliography |
Journal of Microbiology, 56(2),97–103, 2018,
|
DOI |
10.1007/s12275-018-7462-3
|
Key Words |
Lysobacter spongiae, 16S rRNA gene, polyphasic
taxonomy |
Abstract |
A Gram-negative, motile, aerobic and rod-shaped bacterial
strain designated 119BY6-57T was isolated from spongin. The
taxonomic position of the novel isolate was confirmed using
the polyphasic approach. Strain 119BY6-57T grew well at 25–
30°C on marine agar. On the basis of 16S rRNA gene sequence
similarity, strain 119BY6-57T belongs to the family Xanthomonadaceae
and is related to Lysobacter aestuarii S2-CT
(99.8% sequence similarity), L. maris KMU-14T (97.5%), and
L. daejeonensis GH1-9T (97.3%). Lower sequence similarities
(97.0%) were found with all of the other recognized members
of the genus Lysobacter. The G + C content of the genomic
DNA was 69.9 mol%. The major respiratory quinone was Q-8
and the major fatty acids were C16:0 iso, C15:0 iso, summed feature
9 (comprising C17:1 iso ω9c and/or C16:0 10-methyl), summed
feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c), and
C11:0 iso 3-OH. The polar lipids were phosphatidylglycerol,
phosphatidylethanolamine, diphosphatidylglycerol, three unidentified
phospholipids, and an unidentified polar lipid. DNADNA
relatedness values between strain 119BY6-57T and its
closest phylogenetically neighbors were below 48.0 ± 2.1%.
Based on genotypic and phenotypic characteristics, it is concluded
that strain 119BY6-57T is a new member within the genus
Lysobacter, for which the name Lysobacter spongiae sp.
nov. is proposed. The type strain is 119BY6-57T (= KACC
19276T = LMG 30077T). |