Title |
Spirosoma pomorum sp. nov., isolated from apple orchard soil |
Author |
Weilan Li1, Seung-Yeol Lee1, In-Kyu Kang2, Leonid N. Ten1, and Hee-Young Jung1,3* |
Address |
1School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea, 2Department of Horticultural Science, Kyungpook National University, Daegu 41566, Republic of Korea, 3Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Republic of Korea |
Bibliography |
Journal of Microbiology, 56(2),90–96, 2018,
|
DOI |
10.1007/s12275-018-7430-y
|
Key Words |
Spirosoma, Bacteroidetes, polyphasic taxonomy |
Abstract |
A Gram-negative, motile, rod-shaped, aerobic bacterial strain,
designated S7-2-11T, was isolated from apple orchard soil
from Gyeongsangnam-do Province, Republic of Korea, and
was characterized taxonomically using a polyphasic approach.
16S rRNA gene sequence analysis indicated that strain S7-2-
11T belongs to the family Cytophagaceae in phylum Bacteroidetes,
and is closely related to Spirosoma luteolum 16F6ET
(94.2% identity), Spirosoma knui 15J8-12T (92.7%), and Spirosoma
linguale DSM 74T (91.0%). The G + C content of the genomic
DNA of strain S7-2-11T was 49.8 mol%. Strain S7-2-11T
contained summed feature 3 (C16:1 ω7c/C16:1 ω6c; 35.1%), C16:1
ω5c (22.4%), C15:0 iso (13.9%), and C17:0 iso 3-OH (10.6%) as
major cellular fatty acids, and MK-7 as the predominant respiratory
quinone. The main polar lipids were phosphatidylethanolamine,
an unidentified aminophospholipid, and two
unidentified polar lipids. Phenotypic and chemotaxonomic
data supported the affiliation of strain S7-2-11T with the genus
Spirosoma. The results of physiological and biochemical
tests showed the genotypic and phenotypic differentiation of
the isolate from recognized Spirosoma species. On the basis
of its phenotypic properties, genotypic distinctiveness, and
chemotaxonomic features, strain S7-2-11T represents a novel
species of the genus Spirosoma, for which the name Spirosoma
pomorum sp. nov. is proposed. The type strain is S7-2-11T
(= KCTC 52726T = JCM 32130T). |