| Title | Diversity of A mating type in Lentinula edodes and mating type preference in the cultivated strains |
|---|---|
| Author | Byeongsuk Ha1, Sinil Kim1, Minseek Kim1, Yoon Jung Moon1, Yelin Song1, Jae-San Ryu2, Hojin Ryu3, and Hyeon-Su Ro1,4* |
| Address | 1Division of Applied Life Science and Research Institute of Life Sciences, Gyeongsang National University, Jinju 52828, Republic of Korea, 2Department of Mushroom, Korea National College of Agriculture and Fisheries, Jeonju 54874, Republic of Korea, 3Department of Biology, Chungbuk National University, Cheongju 28644, Republic of Korea, 4Division of Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea |
| Bibliography | Journal of Microbiology, 56(6),416–425, 2018, |
| DOI | 10.1007/s12275-018-8030-6 |
| Key Words | diversity, lentinula, mating, nucleus, preference |
| Abstract | Diversity of A mating type in Lentinula edodes has been assessed by analysis of A mating loci in 127 strains collected from East Asia. It was discovered that hypervariable sequence region with an approximate length of 1 kb in the A mating locus, spanning 5region of HD2-intergenic region-5region of HD1, could represent individual A mating type as evidenced by comprehensive mating analysis. The sequence analysis revealed 27 A mating type alleles from 96 cultivated strains and 48 alleles from 31 wild strains. Twelve of them commonly appeared, leaving 63 unique A mating type alleles. It was also revealed that only a few A mating type alleles such as A1, A4, A5, and A7 were prevalent in the cultivated strains, accounting for 62.5% of all A mating types. This implies preferred selection of certain A mating types in the process of strain development and suggests potential role of A mating genes in the expression of genes governing mushroom quality. Dominant expression of an A mating gene HD1 was observed from A1 mating locus, the most prevalent A allele, in A1-containing dikaryons. However, connections between HD1 expression and A1 preference in the cultivated strains remain to be verified. The A mating type was highly diverse in the wild strains. Thirty-six unique A alleles were discovered from relatively small and confined area of mountainous region in Korean peninsula. The number will further increase because no A allele has been recurrently observed in the wild strains and thus newly discovered strain will have good chances to contain new A allele. The high diversity in small area also suggests that the A mating locus has evolved rapidly and thus its diversity will further increase. |