Title |
Antifungal activity of 3-acetylbenzamide produced by actinomycete WA23-4-4 from the intestinal tract of Periplaneta americana |
Author |
Xia Fang1,2, Juan Shen1,2,3, Jie Wang1,2, Zhi-li Chen1,2, Pei-bin lin1,2, Zhi-yu Chen1,2, Lin-yan Liu1,2, Huan-xiong Zeng1,2, and Xiao-bao Jin1,2* |
Address |
1School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou 510006, P. R. China, 2Guangdong Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou 510006, P. R. China, 3School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, P. R. China |
Bibliography |
Journal of Microbiology, 56(7),516–523, 2018,
|
DOI |
10.1007/s12275-018-7510-z
|
Key Words |
Periplaneta americana, actinomycetes WA23-4-4,
Streptomyces, 3-acetyl benzoyl amide, antifungal activity |
Abstract |
Actinomycetes are well-known for producing numerous bioactive
secondary metabolites. In this study, primary screening
by antifungal activity assay found one actinomycete strain
WA23-4-4 isolated from the intestinal tract of Periplaneta
americana that exhibited broad spectrum antifungal activity.
16S rDNA gene analysis of strain WA23-4-4 revealed close
similarity to Streptomyces nogalater (AB045886) with 86.6%
sequence similarity. Strain WA23-4-4 was considered as a
novel Streptomyces and the 16s rDNA sequence has been
submitted to GenBank (accession no. KX291006). The maximum
antifungal activity of WA23-4-4 was achieved when
culture conditions were optimized to pH 8.0, with 12% inoculum
concentration and 210 ml ISP2 medium, which remained
stable between the 5th and the 9th day. 3-Acetyl benzoyl
amide was isolated by ethyl acetate extraction of WA23-
4-4 fermentation broth, and its molecular formula was determined
as C9H9NO2 based on MS, IR, 1H, and 13C NMR
analyses. The compound showed significant antifungal activity
against Candida albicans ATCC 10231 (MIC: 31.25
μg/ml) and Aspergillus niger ATCC 16404 (MIC: 31.25 μg/ml).
However, the compound had higher MIC values against
Trichophyton rubrum ATCC 60836 (MIC: 500 μg/ml) and
Aspergillus fumigatus ATCC 96918 (MIC: 1,000 μg/ml). SEM
analysis showed damage to the cell membrane of Candida
albicans ATCC 10231 and to the mycelium of Aspergillus niger
ATCC 16404 after being treatment with 3-acetyl benzoyl
amide. In conclusion, this is the first time that 3-acetyl benzoyl
amide has been identified from an actinomycete and
this compound exhibited antifungal activity against Candida
albicans ATCC 10231 and Aspergillus niger ATCC 16404. |