Title |
Hymenobacter jeollabukensis sp. nov., isolated from soil |
Author |
Leonid N. Ten1, Young Eun Han1, Kyeung Il Park2, In-Kyu Kang3, Jeung-Sul Han3*, and Hee-Young Jung1,4* |
Address |
1School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea, 2Department of Horticulture and Life Science, Yeungnam University, Gyeongsan 38541, Republic of Korea, 3Department of Horticultural Science, Kyungpook National University, Daegu 41566, Republic of Korea, 4Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Republic of Korea |
Bibliography |
Journal of Microbiology, 56(7),500–506, 2018,
|
DOI |
10.1007/s12275-018-8085-4
|
Key Words |
Hymenobacter, Bacteroidetes, soil bacteria |
Abstract |
A Gram-stain-negative, non-motile, rod-shaped, aerobic bacterial
strain, designated 1-3-3-8T, was isolated from soil and
characterized taxonomically using a polyphasic approach.
Comparative 16S rRNA gene sequence analysis showed that
strain 1-3-3-8T belongs to the family Cytophagaceae of phylum
Bacteroidetes and is most closely related to Hymenobacter
paludis KBP-30T (96.8% similarity), Hymenobacter ocellatus
Myx2105T (96.8%), Hymenobacter coalescens WW84T (95.6%),
and Hymenobacter deserti ZLB-3T (95.4%). The G + C content
of the genomic DNA of strain 1-3-3-8T was 63.6 mol%. The
isolate contained C15:0 iso (28.4%), summed feature 4 (C17:1
anteiso B/C17:1 iso I; 18.9%), and C15:0 anteiso (17.6%) as major
fatty acids, MK-7 as the predominant respiratory quinone,
and sym-homospermidine as the predominant polyamine.
The major polar lipids were phosphatidylethanolamine and
an unidentified lipid. The phenotypic and chemotaxonomic
data supported the affiliation of strain 1-3-3-8T with the genus
Hymenobacter. The DNA-DNA relatedness between strain
1-3-3-8T and H. paludis KCTC 32237T and H. ocellatus DSM
11117T were 24.5 and 27.4% respectively, clearly showing that
the isolate is not related to them at the species level. Overall,
the novel strain could be differentiated from its phylogenetic
neighbors on the basis of several phenotypic, genotypic, and
chemotaxonomic features. Therefore, strain 1-3-3-8T represents
a novel species of the genus Hymenobacter, for which
the name Hymenobacter jeollabukensis sp. nov. has been proposed.
The type strain is 1-3-3-8T (= KCTC 52741T = JCM
32192T). |