Title |
Proposal of three novel species of soil bacteria, Variovorax ureilyticus, Variovorax rhizosphaerae, and Variovorax robiniae, in the family Comamonadaceae |
Author |
Tuan Manh Nguyen1,2, Ngoc Hoang Trinh1,3, and Jaisoo Kim1* |
Address |
1Department of Life Science, College of Natural Sciences and Engineering, Kyonggi University, Suwon 16227, Republic of Korea, 2Thai Nguyen University of Agriculture and Forestry, Quyet Thang Commune, Thai Nguyen City, Vietnam, 3Thai Nguyen University of Sciences, Tan Thinh Ward, Thai Nguyen City, Vietnam |
Bibliography |
Journal of Microbiology, 56(7),485–492, 2018,
|
DOI |
10.1007/s12275-018-8025-3
|
Key Words |
Variovorax ureilyticus sp. nov., Variovorax rhizosphaerae
sp. nov., Variovorax robiniae sp. nov., taxonomy,
soil bacteria, Comamonadaceae |
Abstract |
Three novel bacterial strains (UCM-2T, UCM-G28T, and
UCM-G35T) were obtained while isolating soil bacteria for
the development of antibiotics. Cells of these strains were
Gram-negative, non-spore forming, motile by means of a
single flagellum, and rod shaped. In all strains, the predominant
isoprenoid quinone was ubiquinone-8 (Q-8). Cells
contained C16:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c),
summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), and C17:0 cyclo
as the major fatty acids, and C10:0 3-OH as the major hydroxy
fatty acid. The polar lipid profiles of the three novel strains
were dominated by diphosphatidylglycerol, phosphatidylethanolamine,
and phosphatidylglycerol. The genomic DNA
G + C contents of strains UCM-2T, UCM-G28T, and UCMG35T
were 67.5, 65.9, and 66.4 mol%, respectively. Phylogenetic
analyses based on 16S rRNA sequences showed that
strain UCM-2T was most closely related to Variovorax soli
NBRC 106424T, whereas strains UCM-G28T and UCM-G35T
were most similar to Variovorax ginsengisoli Gsoil 3165T.
Values indicating DNA-DNA hybridization between the
novel isolates and closely related species in the genus Variovorax
were lower than the 70% cut-off point. These phenotypic,
chemotaxonomic, and phylogenetic data indicate
that the three isolates should be classified as new members
of the genus Variovorax, for which the names Variovorax
ureilyticus sp. nov., Variovorax rhizosphaerae sp. nov., and
Variovorax robiniae sp. nov. are proposed. The type strains
are UCM-2T (= KACC 18899T = NBRC 112306T), UCMG28T
(= KACC 18900T = NBRC 112307T), and UCM-G35T
(= KACC 18901T = NBRC 112308T), respectively. |