Title |
Bacillus ferrooxidans sp. nov., an iron(II)-oxidizing bacterium isolated from paddy soil |
Author |
Guo-Wei Zhou1,2, Xiao-Ru Yang2*, Jian-Qiang Su2, Bang-Xiao Zheng2,3, and Yong-Guan Zhu1,2 |
Address |
1State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, P. R. China, 2Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, P. R. China, 3University of Chinese Academy of Sciences, Beijing 100049, P. R. China |
Bibliography |
Journal of Microbiology, 56(7),472–477, 2018,
|
DOI |
10.1007/s12275-018-7543-3
|
Key Words |
Bacillus ferrooxidans, novel species, iron(II)-oxidizing
bacteria, polyphasic taxonomy, paddy soil |
Abstract |
An endospore-forming bacterium, designated YT-3T, was
isolated from a paddy soil in Yingtan, Jiangxi, China. Cells of
strain YT-3T were Gram-positive, rod-shaped, facultative
anaerobic, catalase, and oxidase positive. The optimum growth
temperature and pH were 30°C (ranged from 15 to 50°C)
and 6.5–7.0 (ranged from 3 to 11), respectively. Analysis of
the 16S rRNA gene sequence showed that strain YT-3T was
affiliated to the genus Bacillus and displayed the highest similarity
to that of Bacillus drentensis JCM 21707T (98.3%),
followed by B. ginsengisoli JCM 17335T (97.8%) and B. fumarioli
JCM 21708T (97.0%). The similarity of rpoB gene
sequence between strain YT-3T and B. drentensis JCM 21707T,
B. ginsengisoli JCM 17335T and B. fumarioli JCM 21708T was
80.4%, 81.5%, and 82.1%, respectively. The genomic DNA
G + C content was 44.9 mol%. The predominant respiratory
quinone was Menaquinone-7, and meso-diaminopimelic acid
was present in the peptidoglycan layer of cell wall. The major
fatty acids were C15:0 anteiso (36.2%), C14:0 iso (19.6%),
C15:0 iso (17.4%), and C16:0 iso (9.8%). The polar lipid profile
consisted of diphosphatidylglycerol, phosphatidylethanolamine,
phosphatidylglycerol, phospholipids, and ammoniac
phospholipids. The DNA-DNA hybridization values
between isolate YT-3T and B. drentensis (JCM 21707T), B.
ginsengisoli (JCM 17335T), and B. fumarioli (JCM 21708T)
were 36.3%, 30.3%, and 25.3%, respectively. On the basis of
physiological, genetic and biochemical data, strain YT-3T
represented a novel species of the genus Bacillus, for which
the name Bacillus ferrooxidans sp. nov was proposed. The type
strain is YT-3T (= KCTC 33875T = CCTCC AB 2017049T). |