| Title | Taxonomic description and draft genome of Pseudomonas sediminis sp. nov., isolated from the rhizospheric sediment of Phragmites karka |
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| Author | Pratiksha Behera1,2, Madhusmita Mahapatra1, Arman Seuylemezian3, Parag Vaishampayan3, V. Venkata Ramana4, Neetha Joseph4, Amaraja Joshi4, Yogesh Shouche4, Mrutyunjay Suar2, Ajit K. Pattnaik1, and Gurdeep Rastogi1* |
| Address | 1Wetland Research and Training Centre, Chilika Development Authority, Balugaon 752030, Odisha, India, 2School of Biotechnology, KIIT University, Bhubaneswar 751024, Odisha, India, 3Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, NASA, California Institute of Technology, Pasadena, CA 91109, USA, 4National Centre for Microbial Resource, National Centre for Cell Science, Pune, Maharashtra 411021, India |
| Bibliography | Journal of Microbiology, 56(7),458–466, 2018, |
| DOI | 10.1007/s12275-018-7549-x |
| Key Words | Phragmites karka, Pseudomonas sediminis sp. nov., polyphasic taxonomy, Chilika |
| Abstract | The taxonomic position of a Gram-stain-negative, rod-shaped bacterial strain, designated PI11T , isolated from the rhizospheric sediment of Phragmites karka was characterized using a polyphasic approach. Strain PI11T could grow optimally at 1.0% NaCl concentration with pH 7.0 at 30°C and was positive for oxidase and catalase but negative for hydrolysis of starch, casein, and esculin ferric citrate. Phylogenetic analysis of 16S rRNA gene sequences indicated that the strain PI11T belonged to the genus Pseudomonas sharing the highest sequence similarities with Pseudomonas indoloxydans JCM 14246T (99.72%), followed by, Pseudomonas oleovorans subsp. oleovorans DSM 1045T (99.29%), Pseudomonas toyotomiensis JCM 15604T (99.15%), Pseudomonas chengduensis DSM 26382T (99.08%), Pseudomonas oleovorans subsp. lubricantis DSM 21016T (99.08%), and Pseudomonas alcaliphila JCM 10630T (99.01%). Experimental DNA-DNA relatedness between strain PI11T and P. indoloxydans JCM 14246T was 49.4%. The draft genome of strain PI11T consisted of 4,884,839 bp. Average nucleotide identity between the genome of strain PI11T and other closely related type strains ranged between 77.25–90.74%. The polar lipid pattern comprised of phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylcholine. The major (> 10%) cellular fatty acids were C18:1 ω6c/ω7c, C16:1 ω6c/ω7c, and C16:0. The DNA G + C content of strain PI11T was 62.4 mol%. Based on the results of polyphasic analysis, strain PI11T was delineated from other closely related type strains. It is proposed that strain PI11T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas sediminis sp. nov. is proposed. The type strain is PI11T (= KCTC 42576T = DSMZ 100245T). |