| Title | Photodynamic antimicrobial activity of new porphyrin derivatives against methicillin resistant Staphylococcus aureus |
|---|---|
| Author | Hüseyin Taşlı1, Ayşe Akbıyık2, Nermin Topaloğlu3, and Vildan Alptüzün4 |
| Address | 1Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Ege University, 35100 Izmir, Turkey, 2Department of Nursing, Faculty of Health Sciences, Izmir Katip Celebi University, 35620 Izmir, Turkey, 3Department of Biomedical Engineering, Faculty of Engineering and Architecture Izmir Katip Celebi University, 35620 Izmir, Turkey, 4Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ege University, 35100 Izmir, Turkey |
| Bibliography | Journal of Microbiology, 56(11),828–837, 2018, |
| DOI | 10.1007/s12275-018-8244-7 |
| Key Words | antimicrobial photodynamic therapy, cationic porphyrins, multidrug resistant, Staphylococcus aureus, MRSA, wound infections |
| Abstract | Methicillin resistant Staphylococcus aureus (MRSA) with multiple drug resistance patterns is frequently isolated from skin and soft tissue infections that are involved in chronic wounds. Today, difficulties in the treatment of MRSA associated infections have led to the development of alternative approaches such as antimicrobial photodynamic therapy. This study aimed to investigate photoinactivation with cationic porphyrin derivative compounds against MRSA in in-vitro conditions. In the study, MRSA clinical isolates with different antibiotic resistance profiles were used. The newly synthesized cationic porphyrin derivatives (PM, PE, PPN, and PPL) were used as photosensitizer, and 655 nm diode laser was used as light source. Photoinactivation experiments were performed by optimizing energy doses and photosensitizer concentrations. In photoinactivation experiments with different energy densities and photosensitizer concentrations, more than 99% reduction was achieved in bacterial cell viability. No decrease in bacterial survival was observed in control groups. It was determined that there was an increase in photoinactivation efficiency by increasing the energy dose. At the energy dose of 150 J/cm2 a survival reduction of over 6.33 log10 was observed in each photosensitizer type. While 200 μM PM concentration was required for this photoinactivation, 12.50 μM was sufficient for PE, PPN, and PPL. In our study, antimicrobial photodynamic therapy performed with cationic porphyrin derivatives was found to have potent antimicrobial efficacy against multidrug resistant S. aureus which is frequently isolated from wound infections. |