| Title | Water-based extracts of Zizania latifolia inhibit Staphylococcus aureus infection through the induction of human beta-defensin 2 expression in HaCaT cells |
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| Author | Bo Yeon Kang1, Seung-Su Lee2, Myun-Ho Bang2, Hyoik Jeon1, Hangeun Kim2*, and Dae Kyun Chung1,2* |
| Address | 1Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 17104, Republic of Korea, 2Skin Biotechnology Center, Kyung Hee University, Yongin 17104, Republic of Korea |
| Bibliography | Journal of Microbiology, 56(12),910–916, 2018, |
| DOI | 10.1007/s12275-018-8307-9 |
| Key Words | Zizania latifolia, human beta-defensin 2, Staphylococcus aureus, Toll-like receptor, bacterial infection, antimicrobial |
| Abstract | Zizania latifolia is a perennial herb belonging to the family Gramineae that has been used as a health food in Asian countries. In this study, we investigated the antimicrobial effect of Z. latifolia, which increased human beta-defensin 2 (hBD2) expression in HaCaT cells. hBD2 expression was further increased in cells treated with Z. latifolia extracts and subsequently infected with Staphylococcus aureus. Inversely, S. aureus infection decreased after treatment. The induction of hBD2 in HaCaT cells was mediated by the Toll-like receptor 2 (TLR2) signaling pathway, including the activation of extracellular signal-regulated kinase (ERK) and activator protein 1 (AP-1). Further study using siRNA revealed that hBD2 played an important role in the inhibition of S. aureus infection in HaCaT cells. Our data suggest that Z. latifolia extracts can be used as an antimicrobial ingredient for skin treatment formulas. |