| Title | Paenibacillus seodonensis sp. nov., isolated from a plant of the genus Campanula |
|---|---|
| Author | Myung-Suk Kang1, Ki-Eun Lee2, Eun-Young Lee2, and Soo-Je Park3* |
| Address | 1Biological Resources Utilization Department, National Institute of Biological Resources, Incheon 22689, Republic of Korea, 2Microorganism Resources Division, National Institute of Biological Resources, Incheon 22689, Republic of Korea, 3Department of Biology, Jeju National University, Jeju 63243, Republic of Korea |
| Bibliography | Journal of Microbiology, 56(12),874–879, 2018, |
| DOI | 10.1007/s12275-018-8455-y |
| Key Words | Paenibacillus seodonensis sp. nov., Dokdo, Campanula, polyphasic, novel species |
| Abstract | Strain DCT-19T, representing a Gram-stain-positive, rodshaped, aerobic bacterium, was isolated from a native plant belonging to the genus Campanula on Dokdo, the Republic of Korea. Comparative analysis of the 16S rRNA gene sequence showed that this strain was closely related to Paenibacillus amylolyticus NRRL NRS-290T (98.6%, 16S rRNA gene sequence similarity), Paenibacillus tundrae A10bT (98.1%), and Paenibacillus xylanexedens NRRL B-51090T (97.6%). DNADNA hybridization indicated that this strain had relatively low levels of DNA-DNA relatedness with P. amylolyticus NRRL NRS-290T (30.0%), P. xylanexedens NRRL B-51090T (29.0%), and P. tundrae A10bT (24.5%). Additionally, the genomic DNA G + C content of DCT-19T was 44.8%. The isolated strain grew at pH 6.0–8.0 (optimum, pH 7.0), 0–4% (w/v) NaCl (optimum, 0%), and a temperature of 15–45°C (optimum 25–30°C). The sole respiratory quinone in the strain was menaquinone-7, and the predominant fatty acids were C15:0 anteiso, C16:0 iso, and C16:0. In addition, the major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. Based on its phenotypic properties, genotypic distinctiveness, and chemotaxonomic features, strain DCT-19T is proposed as a novel species in the genus Paenibacillus, for which the name Paenibacillus seodonensis sp. nov. is proposed (=KCTC 43009T =LMG 30888T). The type strain of Paenibacillus seodonensis is DCT-19T. |