Title Lytic KFS-SE2 phage as a novel bio-receptor for Salmonella Enteritidis detection
Author In Young Choi1, Cheonghoon Lee2,3, Won Keun Song1, Sung Jae Jang2, and Mi-Kyung Park1,4*
Address 1School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea, 2Department of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, Seoul 08826, Republic of Korea, 3Institute of Health and Environment, Seoul National University, Seoul 08826, Republic of Korea, 4Food and Bio-industry Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea
Bibliography Journal of Microbiology, 57(2),170–179, 2019,
DOI 10.1007/s12275-019-8610-0
Key Words bio-receptor, phage, Salmonella Enteritidis, lytic, biosensor
Abstract Since Salmonella Enteritidis is one of the major foodborne pathogens, on-site applicable rapid detection methods have been required for its control. The purpose of this study was to isolate and purify S. Enteritidis-specific phage (KFS-SE2 phage) from an eel farm and to investigate its feasibility as a novel, efficient, and reliable bio-receptor for its employment. KFS-SE2 phage was successfully isolated at a high concentration of (2.31 ± 0.43) × 1011 PFU/ml, and consisted of an icosahedral head of 65.44 ± 10.08 nm with a non-contractile tail of 135.21 ± 12.41 nm. The morphological and phylogenetic analysis confirmed that it belongs to the Pis4avirus genus in the family of Siphoviridae. KFS-SE2 genome consisted of 48,608 bp with 45.7% of GC content. Genome analysis represented KFS-SE2 to have distinctive characteristics as a novel phage. Comparative analysis of KFS-SE2 phage with closely related strains confirmed its novelty by the presence of unique proteins. KFS-SE2 phage exhibited excellent specificity to S. Enteritidis and was stable under the temperature range of 4 to 50°C and pH of 3 to 11 (P < 0.05). The latent time was determined to be 20 min. Overall, a new lytic KFS-SE2 phage was successfully isolated from the environment at a high concentration and the excellent feasibility of KFS-SE2 phage was demonstrated as a new bio-receptor for S. Enteritidis detection.