Title |
Oxygen-mediated growth enhancement of an obligate anaerobic archaeon Thermococcus onnurineus NA1 |
Author |
Seong Hyuk Lee1, Hwan Youn2, Sung Gyun Kang1,3*, and Hyun Sook Lee1,3* |
Address |
1Korea Institute of Ocean Science and Technology, Busan 49111, Republic of Korea, 2Department of Biology, California State University, Fresno, CA 93740-8034, USA, 3Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon 34113, Republic of Korea |
Bibliography |
Journal of Microbiology, 57(2),138–142, 2019,
|
DOI |
10.1007/s12275-019-8592-y
|
Key Words |
oxygen, obligate anaerobic archaeon, Thermococcus
onnurineus NA1, transcriptome analysis, antioxidant enzymes |
Abstract |
Thermococcus onnurineus NA1, an obligate anaerobic hyperthermophilic
archaeon, showed variable oxygen (O2) sensitivity
depending on the types of substrate employed as an
energy source. Unexpectedly, the culture with yeast extract
as a sole energy source showed enhanced growth by 2-fold
in the presence of O2. Genome-wide transcriptome analysis
revealed the upregulation of several antioxidant-related genes
encoding thioredoxin peroxidase (TON_0862), rubrerythrin
(TON_0864), rubrerythrin-related protein (TON_0873),
NAD(P)H rubredoxin oxidoreductase (TON_0865), or thioredoxin
reductase (TON_1603), which can couple the detoxification
of reactive oxygen species with the regeneration
of NAD(P)+ from NAD(P)H. We present a plausible mechanism
by which O2 serves to maintain the intracellular redox
balance. This study demonstrates an unusual strategy of an
obligate anaerobe underlying O2-mediated growth enhancement
despite not having heme-based or cytochrome-type
proteins. |