Title Oxygen-mediated growth enhancement of an obligate anaerobic archaeon Thermococcus onnurineus NA1
Author Seong Hyuk Lee1, Hwan Youn2, Sung Gyun Kang1,3*, and Hyun Sook Lee1,3*
Address 1Korea Institute of Ocean Science and Technology, Busan 49111, Republic of Korea, 2Department of Biology, California State University, Fresno, CA 93740-8034, USA, 3Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon 34113, Republic of Korea
Bibliography Journal of Microbiology, 57(2),138–142, 2019,
DOI 10.1007/s12275-019-8592-y
Key Words oxygen, obligate anaerobic archaeon, Thermococcus onnurineus NA1, transcriptome analysis, antioxidant enzymes
Abstract Thermococcus onnurineus NA1, an obligate anaerobic hyperthermophilic archaeon, showed variable oxygen (O2) sensitivity depending on the types of substrate employed as an energy source. Unexpectedly, the culture with yeast extract as a sole energy source showed enhanced growth by 2-fold in the presence of O2. Genome-wide transcriptome analysis revealed the upregulation of several antioxidant-related genes encoding thioredoxin peroxidase (TON_0862), rubrerythrin (TON_0864), rubrerythrin-related protein (TON_0873), NAD(P)H rubredoxin oxidoreductase (TON_0865), or thioredoxin reductase (TON_1603), which can couple the detoxification of reactive oxygen species with the regeneration of NAD(P)+ from NAD(P)H. We present a plausible mechanism by which O2 serves to maintain the intracellular redox balance. This study demonstrates an unusual strategy of an obligate anaerobe underlying O2-mediated growth enhancement despite not having heme-based or cytochrome-type proteins.