Title |
Chryseolinea soli sp. nov., isolated from soil |
Author |
Shin Ae Lee, Yiseul Kim, Mee-Kyung Sang, Jaekyeong Song, Soon-Wo Kwon, and Hang-Yeon Weon* |
Address |
Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea |
Bibliography |
Journal of Microbiology, 57(2),122–126, 2019,
|
DOI |
10.1007/s12275-019-8562-4
|
Key Words |
Chryseolinea, strain KIS68-18, novel species, polyphasic
taxonomy |
Abstract |
A yellow-colored bacterium with gliding motility, strain
KIS68-18T, was isolated from a soil sample at Bijin Island in
Tongyeong city, Republic of Korea. The cells were strictly
aerobic, Gram-staining-negative, non-spore-forming, and
rod-shaped. The strain grew at the range of 10–35°C
(optimum, 25–30°C), pH 5.5–8.0 (optimum, 6.0–7.5), and
0–0.5% (w/v) NaCl. A phylogenetic analysis based on 16S
rRNA gene sequences revealed that strain KIS68-18T was
closely related to Chryseolinea serpens DSM 24574T (98.9%)
and had low sequence similarities (below 92.6%) with other
members of the family ‘Cytophagaceae’ in the phylum
Bacteroidetes. The major respiratory quinone system was
MK-7 and the predominant cellular fatty acids were C16:1
ω5c (38.8%), iso-C15:0 (18.5%), and summed feature 3 (C16:1
ω7c and/or C16:1 ω6c, 10.6%). The polar lipids consisted of
phosphatidylethanolamine, one unidentified phospholipid,
three unidentified aminophospholipids, two unidentified
aminolipids, and five unidentified lipids. The DNA G + C
content was 50.9%. Based on the phylogenetic, physiological,
and chemotaxonomic data, stain KIS68-18T represents
a novel species of the genus Chryseolinea, for which
the name Chryseolinea soli sp. nov. is proposed. The type
strain of Chryseolinea soli is KIS68-18T (= KACC 17327T =
NBRC 113100T). |