Title Blue-Red LED wavelength shifting strategy for enhancing beta-carotene production from halotolerant microalga, Dunaliella salina
Author Sang-Il Han1, Sok Kim1, Changsu Lee2, and Yoon-E Choi1*
Address 1Division of Environmental Science and Ecological Engineering, Korea University, Seoul 02841, Republic of Korea, 2Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea
Bibliography Journal of Microbiology, 57(2),101–106, 2019,
DOI 10.1007/s12275-019-8420-4
Key Words Dunaliella salina, beta-carotene, light-emitting diode, wavelength shift, adaptive laboratory evolution
Abstract In the present study, to improve the photosynthetic betacarotene productivity of Dunaliella salina, a blue-red LED wavelength-shifting system (B-R system) was investigated. Dunaliella salina under the B-R system showed enhanced density and beta-carotene productivity compared to D. salina cultivated under single light-emitting diode light wavelengths (blue, white, and red light-emitting diode). Additionally, we developed blue light-adapted D. salina (ALE-D. salina) using an adaptive laboratory evolution (ALE) approach. In combination with the B-R system applied to ALE-D. salina (ALE B-R system), the beta-carotene concentration (33.94 ± 0.52 μM) was enhanced by 19.7% compared to that observed for the non-ALE-treated wild-type of D. salina (intact D. salina) under the B-R system (28.34 ± 0.24 μM).