| Title | Blue-Red LED wavelength shifting strategy for enhancing beta-carotene production from halotolerant microalga, Dunaliella salina |
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| Author | Sang-Il Han1, Sok Kim1, Changsu Lee2, and Yoon-E Choi1* |
| Address | 1Division of Environmental Science and Ecological Engineering, Korea University, Seoul 02841, Republic of Korea, 2Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea |
| Bibliography | Journal of Microbiology, 57(2),101–106, 2019, |
| DOI | 10.1007/s12275-019-8420-4 |
| Key Words | Dunaliella salina, beta-carotene, light-emitting diode, wavelength shift, adaptive laboratory evolution |
| Abstract | In the present study, to improve the photosynthetic betacarotene productivity of Dunaliella salina, a blue-red LED wavelength-shifting system (B-R system) was investigated. Dunaliella salina under the B-R system showed enhanced density and beta-carotene productivity compared to D. salina cultivated under single light-emitting diode light wavelengths (blue, white, and red light-emitting diode). Additionally, we developed blue light-adapted D. salina (ALE-D. salina) using an adaptive laboratory evolution (ALE) approach. In combination with the B-R system applied to ALE-D. salina (ALE B-R system), the beta-carotene concentration (33.94 ± 0.52 μM) was enhanced by 19.7% compared to that observed for the non-ALE-treated wild-type of D. salina (intact D. salina) under the B-R system (28.34 ± 0.24 μM). |