Title |
Paracoccus jeotgali sp. nov., isolated from Korean salted and fermented shrimp |
Author |
Juseok Kim1,2, Joon Yong Kim3, Hye Seon Song1, In-Tae Cha1, Seong Woon Roh1*, and Se Hee Lee1* |
Address |
1Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea, 2Division of Environmental Science and Ecological Engineering, Korea University, Seoul 02841, Republic of Korea, 3Department of Biology, Kyung Hee University, Seoul 02447, Republic of Korea |
Bibliography |
Journal of Microbiology, 57(6),444–449, 2019,
|
DOI |
10.1007/s12275-019-8704-8
|
Key Words |
Paracoccus jeotgali, polyphasic taxonomy, new
taxon, salted and fermented shrimp |
Abstract |
A Gram-stain-negative and facultatively aerobic bacterium,
designated as strain CBA4604T, was isolated from a traditional
Korean salted and fermented shrimp food (saeu-jeot).
Phylogenetic analysis based on 16S rRNA gene sequences
showed that strain CBA4604T formed a clearly distinct phyletic
lineage from closely related species within the genus
Paracoccus. Strain CBA4604T was the most closely related to
P. koreensis Ch05T (97.5% 16S rRNA gene sequence similarity)
and other type strains (≤ 97.0%). The genome comprised a
chromosome and two plasmids of 3,299,166 bp with 66.5%
G+C content. The DNA-DNA relatedness values between
strain CBA4604T and P. koreensis Ch05T, P. alcaliphilus DSM
8512T, and P. stylophorae KTW-16T were 30.5%, 22.9%, and
16.7%, respectively. Cells of the strain were short rod-shaped
and oxidase- and catalase-positive. The growth of strain CBA-
4604T was observed at 10–40°C (optimum, 37°C), pH 6.0–10.0
(optimum, pH 7.0), and in the presence of 0–8.0% (w/v) NaCl
(optimum, 0–2.0%). Strain CBA4604T contained ubiquinone
10 as the sole isoprenoid quinone and summed feature 8 (C18:1
ω7c/C18:1 ω6c) and C18:0 as the major cellular fatty acids. The
polar lipids consisted of phosphatidylcholine, phosphatidylglycerol,
diphosphatidylglycerol, phospholipid, an unidentified
aminolipid, an unidentified glycolipid, and three unidentified
lipids. Based on its phylogenetic, genomic, phenotypic,
and chemotaxonomic features, we concluded that strain CBA-
4604T represents a novel species in the genus Paracoccus and
we propose the name Paracoccus jeotgali sp. nov. The type
strain is CBA4604T (= KACC 19579T = JCM 32510T). |