Title |
Paradesulfovibrio onnuriensis gen. nov., sp. nov., a chemolithoautotrophic sulfate-reducing bacterium isolated from the Onnuri vent field of the Indian Ocean and reclassification of Desulfovibrio senegalensis as Paradesulfovibrio senegalensis comb. nov. |
Author |
Yun Jae Kim1,2, Jhung-Ahn Yang1, Jae Kyu Lim1,2, Mi-Jeong Park1,2, Sung-Hyun Yang1, Hyun Sook Lee1,2, Sung Gyun Kang1,2, Jung-Hyun Lee1,2, and Kae Kyoung Kwon1,2* |
Address |
1Korea Institute of Ocean Science and Technology, Busan 49111, Republic of Korea, 2KIOST School, University of Science and Technology, Daejeon 34113, Republic of Korea |
Bibliography |
Journal of Microbiology, 58(4),252-259, 2020,
|
DOI |
10.1007/s12275-020-9376-0
|
Key Words |
Paradesulfovibrio onnuriensis, sulfate-reducing
bacteria, deep-sea sediment |
Abstract |
An anaerobic, rod-shaped, mesophilic, chemolithoautotrophic,
sulfate-reducing bacterial strain IOR2T was isolated from
a newly found deep-sea hydrothermal vent (OVF, Onnuri
Vent Field) area in the central Indian Ocean ridge (11°2488
S 66°2542E, 2021 m water depth). The 16S rRNA gene sequence
analysis revealed that the strain IOR2T was most closely
related to Desulfovibrio senegalensis BLaC1T (96.7%).
However, it showed low similarity with the members of the
family Desulfovibrionaceae, such as Desulfovibrio tunisiensis
RB22T (94.0%), D. brasiliensis LVform1T (93.9%), D. halophilus
DSM 5663T (93.7%), and Pseudodesulfovibrio aespoeensis
Aspo-2T (93.2%). The strain IOR2T could grow at 23–
42°C (optimum 37°C), pH 5.0–8.0 (optimum pH 7.0) and
with 0.5–6.5% (optimum 3.0%) NaCl. The strain could use
lactate, pyruvate, H2, and glycerol as electron donors and sulfate,
thiosulfate, and sulfite as electron acceptors. The major
fatty acids of the strain IOR2T were iso-C15:0, iso-C17:0, anteiso-
C15:0, and summed feature 9 (C16:0 methyl/iso-C17:1ω9c).
Both the strains IOR2T and BLaC1T could grow with CO2 and
H2 as the sole sources of carbon and energy, respectively. Genomic
evidence for the Wood-Ljungdahl pathway in both
the strains reflects chemolithoautotrophic growth. The DNA
G + C content of the strain IOR2T and BLaC1T was 58.1–60.5
mol%. Based on the results of the phylogenetic and physiologic
studies, Paradesulfovibrio onnuriensis gen. nov., sp.
nov. with the type strain IOR2T (= KCTC 15845T = MCCC
1K04559T) was proposed to be a member of the family Desulfovibrionaceae.
We have also proposed the reclassification
of D. senegalensis as Paradesulfovibrio senegalensis comb. nov. |