Title |
Limiting the pathogenesis of Salmonella Typhimurium with berry phenolic extracts and linoleic acid overproducing Lactobacillus casei |
Author |
Zajeba Tabashsum1, Mengfei Peng2, Cassendra Bernhardt2, Puja Patel1, Michael Carrion1, Shaik O. Rahaman3, and Debabrata Biswas1,2,4* |
Address |
1Biological Sciences Program - Molecular and Cellular Biology, University of Maryland, College Park, MD, USA, 2Department of Animal and Avian Sciences, University of Maryland, College Park, MD, USA, 3Department of Nutrition and Food Science, University of Maryland, College Park, MD, USA, 4Center for Food Safety and Security Systems, University of Maryland, College Park, MD, USA |
Bibliography |
Journal of Microbiology, 58(6),489–498, 2020,
|
DOI |
10.1007/s12275-020-9545-1
|
Key Words |
prebiotics, probiotics, synbiotics, enteric-pathogen |
Abstract |
The growing threat of emergent multidrug-resistant enteric
bacterial pathogens, and their adopted virulence properties
are directing to find alternative antimicrobials and/or development
of dietaries that can improve host gut health and/or
defense. Recently, we found that modified Lactobacillus casei
(Lc + CLA) with increased production of conjugated linoleic
acid has antimicrobial and other beneficial properties.
Further, prebiotic alike products such as berry pomace extracts
(BPEs), increase the growth of probiotics and inhibit
the growth of certain bacterial pathogens. In this study, we
evaluated the antibacterial effect of genetically modified Lc +
CLA along with BPEs against major enteric pathogen Salmonella
enterica serovar Typhimurium (ST). In mixed culture
condition, the growth of ST was significantly reduced in the
presence of Lc + CLA and/or BPEs. Bacterial cell-free cultural
supernatant (CFCS) collected from wild-type Lc or modified
Lc + CLA strains also inhibited the growth and survival of ST,
and those inhibitory effects were enhanced in the presence of
BPEs. We also found that the interaction of the pathogen with
cultured host (HD-11 and INT-407) cells were also altered in
the presence of either Lc or Lc + CLA strain or their CFCSs
significantly. Furthermore, the relative expression of genes
related to ST virulence and physicochemical properties of ST
was altered by the effect of CFCSs of either Lc or Lc + CLA.
These findings indicate that a diet containing synbiotic, specifically
linoleic acid, over-produced Lc + CLA and prebiotic
product BPEs, might have the potential to be effective in controlling
ST growth and pathogenesis. |