Title |
Aequoribacter fuscus gen. nov., sp. nov., a new member of the family Halieaceae, isolated from coastal seawater |
Author |
Shan-Hui Li, Jaeho Song, Ilnam Kang, Juchan Hwang, and Jang-Cheon Cho* |
Address |
Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea |
Bibliography |
Journal of Microbiology, 58(6),463–471, 2020,
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DOI |
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Key Words |
Aequoribacter fuscus, taxonomy, complete genome,
Halieaceae, coastal sea, marine surface |
Abstract |
A Gram-stain-negative, rod-shaped, obligately aerobic, nonflagellated,
and chemoheterotrophic bacterium, designated
IMCC3088T, was isolated from coastal seawater of the Yellow
Sea. The 16S rRNA gene sequence analysis indicated that
this strain belonged to the family Halieaceae which shared
the highest sequence similarities with Luminiphilus syltensis
NOR5-1BT (94.5%) and Halioglobus pacificus S1-72T (94.5%),
followed by 92.3–94.3% sequence similarities with other species
within the aforementioned family. Phylogenetic analyses
demonstrated that strain IMCC3088T was robustly clustered
with Luminiphilus syltensis NOR5-1BT within the family
Halieaceae. However, average amino acid identity (AAI), percentages
of conserved proteins (POCP), average nucleotide
identity (ANI), and alignment fraction (AF) between strain
IMCC3088T and Luminiphilus syltensis NOR5-1BT were 54.5%,
47.7%, 68.0%, and 16.5%, respectively, suggesting that they
belonged to different genera. Whole-genome sequencing of
strain IMCC3088T revealed a 3.1 Mbp genome size with a
DNA G + C content of 51.7 mol%. The genome encoded diverse
metabolic pathways including sulfur oxidation, phenol
degradation, and proteorhodopsin phototrophy. Mono-unsaturated
fatty acids were found to be the predominant cellular
fatty acid components in the strain. Phosphatidylethanolamine,
phosphatidylglycerol, and diphosphatidylglycerol
were the primarily identified polar lipids, and ubiquinone-8
was identified as a major respiratory quinone. The taxonomic
data collected herein suggested that strain IMCC3088T represented
a novel genus and species of the family Halieaceae,
for which the name Aequoribacter fuscus gen. nov., sp. nov.
is proposed with the type strain (= KACC 15529T = NBRC
108213T). |