Title |
The synergy effect of arbuscular mycorrhizal fungi symbiosis and exogenous calcium on bacterial community composition and growth performance of peanut (Arachis hypogaea L.) in saline alkali soil |
Author |
Dunwei Ci1, Zhaohui Tang2, Hong Ding1, Li Cui3, Guanchu Zhang1, Shangxia Li1, Liangxiang Dai1, Feifei Qin1, Zhimeng Zhang1, Jishun Yang1*, and Yang Xu1* |
Address |
1Shandong Peanut Research Institute, Shandong Academy of Agricultural Sciences, Qingdao, Shandong 266100, P. R. China, 2Biotechnology Research Center, Shandong Academy of Agricultural Sciences/Shandong Key Lab. of Genetic Improvement, Ecology and Physiology of Crops, Jinan 250100, P. R. China, 3Shandong Provincial Crop Germplasm Resource Centre, Shandong Academy of Agricultural Sciences, Jinan 250100, P. R. China |
Bibliography |
Journal of Microbiology, 59(1),51–63, 2021,
|
DOI |
10.1007/s12275-021-0317-3
|
Key Words |
Arachis hypogaea L., 16S rRNA, rhizosphere bacterial
community, saline alkali soil, AMF, Ca2+ |
Abstract |
Peanut (Arachis hypogaea. L) is an important oil seed crop.
Both arbuscular mycorrhizal fungi (AMF) symbiosis and calcium
(Ca2+) application can ameliorate the impact of saline
soil on peanut production, and the rhizosphere bacterial communities
are also closely correlated with peanut salt tolerance;
however, whether AMF and Ca2+ can withstand high-salinity
through or partially through modulating rhizosphere bacterial
communities is unclear. Here, we used the rhizosphere
bacterial DNA from saline alkali soil treated with AMF and
Ca2+ alone or together to perform high-throughput sequencing
of 16S rRNA genes. Taxonomic analysis revealed that
AMF and Ca2+ treatment increased the abundance of Proteobacteria
and Firmicutes at the phylum level. The nitrogenfixing
bacterium Sphingomonas was the dominant genus in
these soils at the genus level, and the soil invertase and urease
activities were also increased after AMF and Ca2+ treatment,
implying that AMF and Ca2+ effectively improved the living
environment of plants under salt stress. Moreover, AMF combined
with Ca2+ was better than AMF or Ca2+ alone at altering
the bacterial structure and improving peanut growth in saline
alkali soil. Together, AMF and Ca2+ applications are conducive
to peanut salt adaption by regulating the bacterial community
in saline alkali soil. |