Title |
Extracellular products-mediated interspecific interaction between Pseudomonas aeruginosa and Escherichia coli |
Author |
Yang Yuan1, Jing Li1, Jiafu Lin1, Wenjuan Pan1, Yiwen Chu1, Balakrishnan Prithiviraj2, Yidong Guo1, Xinrong Wang1*, and Kelei Zhao1* |
Address |
1Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province, Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, Sichuan, P. R. China, 2Marine Bio-products Research Laboratory, Department of Plant, Food and Environmental Sciences, Dalhousie University, Truro B2N 5E3, NS, Canada |
Bibliography |
Journal of Microbiology, 59(1),29–40, 2021,
|
DOI |
10.1007/s12275-021-0478-0
|
Key Words |
Pseudomonas aeruginosa, Escherichia coli, interaction,
transcriptional profile, pyocyanin, virulence |
Abstract |
The Gram-negative pathogen Pseudomonas aeruginosa adopts
several elaborate strategies to colonize a wide range of natural
or clinical niches and to overcome the neighboring bacterial
competitors in polymicrobial communities. However,
the relationship and interaction mechanism of P. aeruginosa
with other bacterial pathogens remains largely unexplored.
Here we explore the interaction dynamics of P. aeruginosa and
Escherichia coli, which frequently coinfect the lungs of immunocompromised
hosts, by using a series of on-plate proximity
assays and RNA-sequencing. We show that the extracellular
products of P. aeruginosa can inhibit the growth of
neighboring E. coli and induce a large-scale of transcriptional
reprogramming of E. coli, especially in terms of cellular respiration-
related primary metabolisms and membrane components.
In contrast, the presence of E. coli has no significant
effect on the growth of P. aeruginosa in short-term culture,
but causes a dysregulated expression of genes positively controlled
by the quorum-sensing (QS) system of P. aeruginosa
during subsequent pairwise culture. We further demonstrate
that the divergent QS-regulation of P. aeruginosa may be related
to the function of the transcriptional regulator PqsR,
which can be enhanced by E. coli culture supernatant to increase
the pyocyanin production by P. aeruginosa in the absence
of the central las-QS system. Moreover, the extracellular
products of E. coli promote the proliferation and lethality
of P. aeruginosa in infecting the Caenorhabditis elegans
model. The current study provides a general characterization
of the extracellular products-mediated interactions between
P. aeruginosa and E. coli, and may facilitate the understanding
of polymicrobial infections. |