Title |
Monthly distribution of ammonia-oxidizing microbes in a tropical bay |
Author |
Tie-Qiang Mao2, Yan-Qun Li2, Hong-Po Dong1*, Wen-Na Yang2, and Li-Jun Hou1 |
Address |
1State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200062, P. R. China, 2School of Ocean and Meteorology, Guangdong Ocean University, Zhanjiang 524088, P. R. China |
Bibliography |
Journal of Microbiology, 59(1),10–19, 2021,
|
DOI |
10.1007/s12275-021-0287-5
|
Key Words |
ammonia-oxidizing microbes, monthly distribution,
community structure, tropical bay |
Abstract |
Ammonia oxidation, performed by ammonia-oxidizing archaea
(AOA) and bacteria (AOB), plays a critical role in the cycle
of nitrogen in the ocean. For now, environmental variables
controlling distribution of ammonia-oxidizing microbes are
still largely unknown in oceanic environments. In this study,
we used real-time quantitative PCR and high-throughput sequencing
methods to investigate the abundance and diversity
of AOA and AOB from sediment and water in Zhanjiang Bay.
Phylogenic analysis revealed that the majority of AOA amoA
sequences in water and sediment were affiliated with the genus
Nitrosopumilus, whereas the Nitrosotalea cluster was only detected
with low abundance in water. Nitrosomonas and Nitrosospira
dominated AOB amoA sequences in water and sediment,
respectively. The amoA copy numbers of both AOA and
AOB varied significantly with month for both sediment and
water. When water and sediment temperature dropped to 17–
20°C in December and February, respectively, the copy number
of AOB amoA genes increased markedly and was much
higher than for AOA amoA genes. Also, AOA abundance in
water peaked in December when water temperature was lowest
(17–20°C). Stepwise multiple regression analyses revealed that
temperature was the most key factor driving monthly changes
of AOA or AOB abundance. It is inferred that low water temperature
may inhibit growth of phytoplankton and other microbes
and so reduce competition for a common substrate,
ammonium. |