Title |
Vagococcus zengguangii sp. nov., isolated from yak faeces |
Author |
Yajun Ge1, Dong Jin1,2,3, Xin-He Lai4, Jing Yang1,2,3, Shan Lu1,2,3, Ying Huang2, Han Zheng2, Xiaoyan Zhang1, and Jianguo Xu1,2,3 |
Address |
1Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, P. R. China, 2State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, P. R. China, 3Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing, P. R. China, 4Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, Shangqiu Normal University, Shangqiu 476000, P. R. China |
Bibliography |
Journal of Microbiology, 59(1),1–9, 2021,
|
DOI |
10.1007/s12275-021-0406-3
|
Key Words |
Vagococcus, novel species, yak, Qinghai-Tibet
Plateau |
Abstract |
Two unknown Gram-stain-positive, catalase- and oxidasenegative,
non-motile, and coccus-shaped bacteria, designated
MN-17T and MN-09, were isolated from yaks faeces (Bos
grunniens) in the Qinghai-Tibet Plateau of China. 16S rRNA
gene sequence-based comparative analyses revealed that the
two strains were grouped within the genus Vagococcus, displaying
the highest similarity with Vagococcus xieshaowenii
CGMCC 1.16436T (98.6%) and Vagococcus elongatus CCUG
51432T (96.4%). Both strains grew optimally at 37°C and pH
7.0 in the presence of 0.5% (w/v) NaCl. The complete genome
of MN-17T comprises 2,085 putative genes with a total
of 2,190,262 bp and an average G + C content of 36.7 mol%.
The major fatty acids were C16:0 (31.2%), C14:0 (28.5%), and
C18:1ω9c (13.0%); the predominant respiratory quinone was
MK-7 (68.8%); the peptidoglycan type was A4α(L-Lys-DAsp);
and the major polar lipid was diphosphatidylglycerol.
Together, these supported the affiliation of strain MN-17T
to the genus Vagococcus. In silico DNA-DNA hybridization
and the average nucleotide identity values between MN-17T
and all recognized species in the genus were 21.6–26.1%
and 70.7–83.0%, respectively. MN-17T produced acid from
D-cellobiose, D-fructose, glycerol, D-glucose, N-acetyl-glucosamine,
gentiobiose, D-mannose, D-maltose, D-ribose, Dsaccharose,
salicin, D-trehalose, and D-xylose. These results
distinguished MN-17T and MN-09 from closely related species
in Vagococcus. Thus, we propose that strains MN-17T
and MN-09 represent a novel species in the genus Vagococcus,
with the name Vagococcus zengguangii sp. The type strain
is MN-17T (= CGMCC 1.16726T = GDMCC 1.1589T = JCM
33478T). |