| Title |
Spot 42 RNA regulates putrescine catabolism in Escherichia coli by controlling the expression of puuE at the post-transcription level |
| Author |
Xin Sun, Ruyan Li, Guochen Wan, Wanli Peng, Shuangjun Lin, Zixin Deng, and Rubing Liang* |
| Address |
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China |
| Bibliography |
Journal of Microbiology, 59(2),175–185, 2021,
|
| DOI |
|
| Key Words |
Small RNA, Spot 42, putrescine, puuE, carbon
catabolite repression |
| Abstract |
Putrescine, a typical polyamine compound important for
cell growth and stress resistance, can be utilized as an energy
source. However, the regulation of its catabolism is unclear.
Here the small RNA (sRNA) Spot 42, an essential regulator
of carbon catabolite repression (CCR), was confirmed to participate
in the post-transcriptional regulation of putrescine
catabolism in Escherichia coli. Its encoding gene spf exclusively
exists in the γ-proteobacteria and contains specific binding
sites to the 5-untranslated regions of the puuE gene, which
encodes transaminase in the glutamylated putrescine pathway
of putrescine catabolism converting γ-aminobutyrate
(GABA) into succinate semialdehyde (SSA). The transcription
of the spf gene was induced by glucose, inhibited by putrescine,
and unaffected by PuuR, the repressor of puu genes.
Excess Spot 42 repressed the expression of PuuE significantly
in an antisense mechanism through the direct and specific
base-pairing between the 51–57 nt of Spot 42 and the 5-
UTR of puuE. Interestingly, Spot 42 mainly influenced the
stability of the puuCBE transcript. This work revealed the regulatory
role of Spot 42 in putrescine catabolism, in the switch
between favorable and non-favorable carbon source utilization,
and in the balance of metabolism of carbon and nitrogen
sources. |
