Title |
Description of Vagococcus coleopterorum sp. nov., isolated from the intestine of the diving beetle, Cybister lewisianus, and Vagococcus hydrophili sp. nov., isolated from the intestine of the dark diving beetle, Hydrophilus acuminatus, and emended description of the genus Vagococcus |
Author |
Dong-Wook Hyun, Euon Jung Tak, Pil Soo Kim, and Jin-Woo Bae |
Address |
Department of Biology and Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 02447, Republic of Korea |
Bibliography |
Journal of Microbiology, 59(2),132–141, 2021,
|
DOI |
10.1007/s12275-021-0485-1
|
Key Words |
Vagococcus, Vagococcus coleopterorum, Vagococcus
hydrophili, diving beetle, Hydrophilus acuminatus, Cybister
lewisianus |
Abstract |
A polyphasic taxonomic approach was used to characterize
two novel bacterial strains, HDW17AT and HDW17BT, isolated
from the intestine of the diving beetle Cybister lewisianus,
and the dark diving beetle Hydrophilus acuminatus,
respectively. Both strains were Gram-positive and facultative
anaerobic cocci forming cream-colored colonies. The isolates
grew optimally at 25°C, pH 7, in the presence of 0.3% (wt/vol)
NaCl. Phylogenetic analysis based on 16S rRNA gene sequences
and genome sequences showed that the isolates were
members of the genus Vagococcus, and strain HDW17AT was
closely related to Vagococcus fessus CCUG 41755T (98.9% of
16S rRNA gene sequence similarity and 74.3% of average
nucleotide identity [ANI]), whereas strain HDW17BT was
closely related to Vagococcus fluvialis NCFB 2497T (98.9% of
16S rRNA gene sequence similarity and 76.6% of ANI). Both
strains contained C16:0, and C18:1 ω9c as the major cellular fatty
acids, but C16:1 ω9c was also observed only in strain HDW17BT
as the major cellular fatty acid. The respiratory quinone of the
isolates was MK-7. The major polar lipid components were
phosphatidylglycerol, phosphatidylethanolamine, and diphosphatidylglycerol.
The genomic DNA G + C content of strains
HDW17AT and HDW17BT were 36.6 and 34.4%, respectively.
Both strains had cell wall peptidoglycan composed of the
amino acids L-alanine, glycine, D-glutamic acid, L-tryptophan,
L-lysine, and L-aspartic acid, and the sugars ribose, glucose,
and galactose. Based on phylogenetic, phenotypic, chemotaxonomic,
and genotypic analyses, strains HDW17AT and
HDW17BT represent two novel species in the genus Vagococcus.
We propose the name Vagococcus coleopterorum sp.
nov. for strain HDW17AT (= KACC 21348T = KCTC 49324T
= JCM 33674T) and the name Vagococcus hydrophili sp. nov.
for strain HDW17BT (= KACC 21349T = KCTC 49325T =
JCM 33675T). |