| Title | Microbial phenomics linking the phenotype to function: The potential of Raman spectroscopy |
|---|---|
| Author | Jin-Kyung Hong, Soo Bin Kim, Eun Sun Lyou, and Tae Kwon Lee |
| Address | Department of Environmental Engineering, Yonsei University, Wonju 26493, Republic of Korea |
| Bibliography | Journal of Microbiology, 59(3),249–258, 2021, |
| DOI | 10.1007/s12275-021-0590-1 |
| Key Words | Raman spectroscopy, phenotype, bacteria, stable isotope, cell sorting |
| Abstract | Raman spectroscopy is a promising tool for identifying microbial phenotypes based on single cell Raman spectra reflecting cellular biochemical biomolecules. Recent studies using Raman spectroscopy have mainly analyzed phenotypic changes caused by microbial interactions or stress responses (e.g., antibiotics) and evaluated the microbial activity or substrate specificity under a given experimental condition using stable isotopes. Lack of labelling and the nondestructive pretreatment and measurement process of Raman spectroscopy have also aided in the sorting of microbial cells with interesting phenotypes for subsequently conducting physiology experiments through cultivation or genome analysis. In this review, we provide an overview of the principles, advantages, and status of utilization of Raman spectroscopy for studies linking microbial phenotypes and functions. We expect Raman spectroscopy to become a next-generation phenotyping tool that will greatly contribute in enhancing our understanding of microbial functions in natural and engineered systems. |