Title |
CagL polymorphisms between East Asian and Western Helicobacter pylori are associated with different abilities to induce IL-8 secretion |
Author |
Yun Hui Choi1†, Jing Lai1†, Myeong-A Kim1, Aeryun Kim1, Jinmoon Kim1, Hanfu Su2*, Linhu Ge2*, and Jeong-Heon Cha1,2* |
Address |
1Department of Oral Biology, Oral Science Research Center, Department of Applied Life Science, the Graduate School, BK21 FOUR Project, Yonsei University College of Dentistry, Seoul 03722, Republic of Korea, 2Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, Guangdong 510182, P. R. China |
Bibliography |
Journal of Microbiology, 59(8),763–770, 2021,
|
DOI |
10.1007/s12275-021-1136-2
|
Key Words |
Helicobacter pylori, type IV secretion system, CagL,
polymorphism |
Abstract |
Helicobacter pylori colonizes human gastric mucosa. Its infection
is associated with gastric diseases including gastric
cancer. CagA is one of the most important toxins produced
by H. pylori. It is related to gastric cancer which can be injected
into host cells via a type IV secretion system (T4SS).
CagL is a structural component of T4SS apparatus, which
triggers host cell signaling pathway. It has been reported that
CagL polymorphisms may influence the severity of disease
development. To explore the contribution of CagL polymorphisms
between East Asian and Western H. pylori in pathogenesis,
cagL gene in G27 H. pylori was swapped by K74 cagL
which is identical to East Asian CagL consensus sequence and
by Western 26695 H. pylori, resulting in G27ΔcagL/cagLK74
and G27ΔcagL/cagL26695, respectively. Intriguingly, G27ΔcagL/
cagLK74 showed significantly less ability of IL-8 induction
than G27ΔcagL/cagL26695 while displayed similar abilities of
CagA phosphorylation, and cell elongation. Taken together,
this study suggests that the CagL polymorphism may influence
IL-8 induction, and K74 CagL has less ability to induce
IL-8 secretion than G27 or 26695 CagL. Further research
should address how the different capabilities of IL-8 induction
between intraspecies-CagL are associated with the large
differences of the incidence of gastric cancer between East
Asian and Western countries. |