Title |
Fus3 and Tpk2 protein kinases regulate the phosphorylation-dependent functions of RNA helicase Dhh1 in yeast mating and Ste12 protein expression |
Author |
Jaehee Hwang, Daehee Jung, and Jinmi Kim* |
Address |
Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 34134, Republic of Korea |
Bibliography |
Journal of Microbiology, 60(8),843-848, 2022,
|
DOI |
10.1007/s12275-022-2213-x
|
Key Words |
Dhh1, Ste12 expression, Phosphorylation, Dhh1-
Puf6 interaction, Fus3 MAP kinase, TPK2 kinase |
Abstract |
Decapping of mRNA is a key regulatory step for mRNA decay
and translation. The RNA helicase, Dhh1, is known as a
decapping activator and translation repressor in yeast Saccharomyces
cerevisiae. Dhh1 also functions as a gene-specific
positive regulator in the expression of Ste12, a mating-specific
transcription factor. A previous study showed that the Nerminal
phosphorylation of Dhh1 regulates its association
with the mRNA-binding protein, Puf6, to affect the protein
translation of Ste12. Here, we investigated the roles of the
phosphorylated residues of Dhh1 in yeast mating process and
Ste12 expression. The phospho-deficient mutation, DHH1-
T10A, was associated with decreased diploid formation during
mating and decreased level of the Ste12 protein in response
to α-mating pheromone. A kinase overexpression analysis
revealed that Ste12 protein expression was affected by
overexpression of Fus3 MAP kinase or Tpk2 kinase. Tpk2
was shown to be responsible for phosphorylation of Dhh1 at
Thr10. Our study shows that overexpression of Fus3 or Tpk2
alters the Dhh1-Puf6 protein interaction and thereby affects
Ste12 protein expression. |