Title |
Flavihumibacter fluminis sp. nov. and Flavihumibacter rivuli sp. nov., isolated from a freshwater stream |
Author |
Miri S. Park1, Hyeonuk Sa1, Ilnam Kang2*, and Jang-Cheon Cho1* |
Address |
1Department of Biological Sciences and Bioengineering, Inha University, Incheon 22212, Republic of Korea, 2Department of Biological Sciences, Center for Molecular and Cell Biology, Inha University, Incheon 22212, Republic of Korea |
Bibliography |
Journal of Microbiology, 60(8),806-813, 2022,
|
DOI |
10.1007/s12275-022-2298-2
|
Key Words |
Flavihumibacter fluminis, Flavihumibacter rivuli,
new species, polyphasic taxonomy, freshwater |
Abstract |
Two Gram-stain-positive, aerobic, chemoheterotrophic, nonmotile,
rod-shaped, and yellow-pigmented bacterial strains,
designated IMCC34837T and IMCC34838T, were isolated from
a freshwater stream. Results of 16S rRNA gene-based phylogenetic
analyses showed that strains IMCC34837T and IMCC-
34838T shared 96.3% sequence similarity and were most closely
related to Flavihumibacter profundi Chu64-6-1T (99.6%)
and Flavihumibacter cheonanensis WS16T (96.4%), respectively.
Complete whole-genome sequences of strains IMCC-
34837T and IMCC34838T were 5.0 Mbp and 4.3 Mbp of genome
size with 44.5% and 47.9% of DNA G + C contents,
respectively. Average nucleotide identity (ANI) and digital
DNA- DNA hybridization (dDDH) values between the two
strains were 70.0% and 17.9%, repectively, revealing that they
are independent species. The two strains showed ≤ 75.2% ANI
and ≤ 19.3% dDDH values to each closely related species of the
genus Flavihumibacter, indicating that the two strains represent
each novel species. Major fatty acid constituents of
strain IMCC34837T were iso-C15:0, iso-C15:1 G and anteiso-C15:0
and those of strain IMCC34838T were iso-C15:0 and iso-C15:1
G. The predominant isoprenoid quinone detected in both
strains was menaquinone-7 (MK-7). Major polar lipids of
both strains were phosphatidylethanolamine, aminolipids,
and glycolipids. Based on the phylogenetic and phenotypic
characterization, strains IMCC34837T and IMCC34838T were
considered to represent two novel species within the genus
Flavihumibacter, for which the names Flavihumibacter fluminis
sp. nov. and Flavihumibacter rivuli sp. nov. are proposed
with IMCC34837T (= KACC 21752T = NBRC 115292T)
and IMCC34838T (= KACC 21753T = NBRC 115293T) as
the type strains, respectively. |