Title |
Correlation between fat accumulation and fecal microbiota in crossbred pigs |
Author |
Xin Li, Mengyu Li, Jinyi Han, Chuang Liu, Xuelei Han, Kejun Wang, Ruimin Qiao, Xiu-Ling Li*, and Xin-Jian Li* |
Address |
College of Animal Sciences and Technology, Henan Agricultural University, Zhengzhou, Henan 450046, P. R. China |
Bibliography |
Journal of Microbiology, 60(11),1077-1085, 2022,
|
DOI |
10.1007/s12275-022-2218-5
|
Key Words |
backfat thickness, fecal microbiota, 16S rRNA,
pig |
Abstract |
Backfat thickness (BF) is an important indicator of fat deposition
capacity and lean meat rate in pigs and is very important
in porcine genetics and breeding. Intestinal microbiota
plays a key role in nutrient digestion and utilization with a
profound impact on fat deposition of livestock animals. To
investigate the relationship between the pig gut microbiome
and BF, 20 low-BF (L-BF) and 20 high-BF (H-BF) pigs were
selected as two groups from Yunong Black pigs in the present
study. Fecal samples from pigs were analyzed for microbial
diversity, composition, and predicted functionality using 16S
rRNA gene sequencing. The results showed that there were
significant differences in microbial β diversity between the
two groups. LEfSe analysis revealed a number of bacterial features
being differentially enriched in either L-BF or H-BF pigs.
Spearman correlation analysis identified the abundance of
Oscillospira, Peptococcus, and Bulleidia were significantly
positive correlations with BF (P < 0.05), while Sutterella and
Bifidobacterium were significantly negatively correlated with
BF (P < 0.05). Importantly, the bacteria significantly positively
correlated with BF mainly belong to Clostridium, which can
ferment host-indigestible plant polysaccharides into shortchain
fatty acid (SCFA) and promote fat synthesis and deposition.
Predictive functional analysis indicated that the pathway
abundance of cell motility and glycan biosynthesis were
significantly widespread in the microbiota of the H-BF group.
The results of this study will be useful for the development of
microbial biomarkers for predicting and improving porcine
BF, as well as for the investigation of targets for dietary strategies. |