| Title | Rasiella rasia gen. nov. sp. nov. within the family Flavobacteriaceae isolated from seawater recirculating aquaculture system |
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| Author | Seong-Jin Kim1, Young-Sam Kim2, Sang-Eon Kim1,3, Hyun-Kyoung Jung2, Jeeeun Park1,3, Min-Ju Yu1, and Kyoung-Ho Kim1,3* |
| Address | 1Department of Microbiology, Pukyong National University, Busan 48513, Republic of Korea, 2Biotechnology Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea, 3School of Marine and Fisheries Life Science, Pukyong National University, Busan 48513, Republic of Korea |
| Bibliography | Journal of Microbiology, 60(11),1070-1076, 2022, |
| DOI | 10.1007/s12275-022-2099-7 |
| Key Words | Rasiella rasia, Flavobacteriaceae, seawater recirculating aquaculture system, RAS, new genus |
| Abstract | A novel bacterium designated RR4-40T was isolated from a biofilter of seawater recirculating aquaculture system in Busan, South Korea. Cells are strictly aerobic, Gram-negative, irregular short rod, non-motile, and oxidase- and catalase-negative. Growth was observed at 15–30°C, 0.5–6% NaCl (w/v), and pH 5.0–9.5. The strain grew optimally at 28°C, 3% salinity (w/v), and pH 8.5. The phylogenetic analysis based on 16S rRNA gene sequences showed that strain RR4-40T was most closely related to Marinirhabdus gelatinilytica NH83T (94.16% of 16S rRNA gene similarity) and formed a cluster with genera within the family Flavobacteriaceae. The values of the average nucleotide identity (ANI), digital DNA-DNA hybridization (dDDH), and average amino acid identity (AAI) between genomes of strain RR4-40T and M. gelatinilytica NH83T were 72.91, 18.2, and 76.84%, respectively, and the values against the strains in the other genera were lower than those. The major fatty acids were iso-C15:0 (31.34%), iso-C17:0 3-OH (13.65%), iso-C16:0 3-OH (10.61%), and iso-C15:1 G (10.38%). The polar lipids comprised phosphatidylglycerol, diphosphatidylglycerol, aminophospholipid, aminolipid, glycolipid, and sphingolipid. The major respiratory quinone was menaquinone-6 (MK-6) and the DNA G + C content of strain RR4-40T was 37.4 mol%. According to the polyphasic analysis, strain RR4-40T is considered to represent a novel genus within the family Flavobacteriaceae, for which the name Rasiella rasia gen. nov, sp. nov. is proposed. The type strain is RR4-40T (= KCTC 52650T = MCCC 1K04210T). |