Title |
Transcript-specific selective translation by specialized ribosomes bearing genome-encoded heterogeneous rRNAs in V. vulnificus CMCP6 |
Author |
Younkyung Choi1, Minju Joo1, Wooseok Song1, Minho Lee2, Hana Hyeon1, Hyun-Lee Kim1, Ji-Hyun Yeom1, Kangseok Lee1*, and Eunkyoung Shin1* |
Address |
1Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea, 2Department of Microbiology, College of Medicine, Hallym University, Chuncheon 24252, Republic of Korea |
Bibliography |
Journal of Microbiology, 60(12),1162-1167, 2022,
|
DOI |
10.1007/s12275-022-2437-9
|
Key Words |
ribosome, heterogenous rRNAs, specialized ribosomes,
V. vulnificus CMCP6, pathogenic bacteria |
Abstract |
Ribosomes composed of genome-encoded heterogeneous
rRNAs are implicated in the rapid adaptation of bacterial
cells to environmental changes. A previous study showed that
ribosomes bearing the most heterogeneous rRNAs expressed
from the rrnI operon (I-ribosomes) are implicated in the preferential
translation of a subset of mRNAs, including hspA
and tpiA, in Vibrio vulnificus CMCP6. In this study, we show
that HspA nascent peptides were predominantly bound to
I-ribosomes. Specifically, I-ribosomes were enriched more
than two-fold in ribosomes that were pulled down by immunoprecipitation
of HspA peptides compared with the proportion
of I-ribosomes in crude ribosomes and ribosomes pulled
down by immunoprecipitation of RNA polymerase subunit
ß peptides in the wild-type (WT) and rrnI-completed strains.
Other methods that utilized the incorporation of an affinity
tag in 23S rRNA or chimeric rRNA tethering 16S and 23S
rRNAs, which generated specialized functional ribosomes
in Escherichia coli, did not result in functional I-ribosomes
in V. vulnificus CMCP6. This study provides direct evidence
of the preferential translation of hspA mRNA by I-ribosomes. |