| Title | Secretions from Serratia marcescens Inhibit the Growth and Biofilm Formation of Candida spp. and Cryptococcus neoformans |
|---|---|
| Author | Caiyan Xin1, Fen Wang2, Jinping Zhang1,3, Quan Zhou1, Fangyan Liu1, Chunling Zhao1*, and Zhangyong Song1,3* |
| Address | 1School of Basic Medical Science, Southwest Medical University, Luzhou 646000, People’s Republic of China, 2The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou 646000, People’s Republic of China, 3Technical Platform for the Molecular Biology, Research Core Facility, Southwest Medical University, Luzhou 646000, People’s Republic of China |
| Bibliography | Journal of Microbiology, 61(2),221-232, 2023, |
| DOI | 10.1007/s12275-022-00007-3 |
| Key Words | Antifungal agents · Candida · Cryptococcus · Culture supernatant · Serratia marcescens |
| Abstract | Candida spp. and Cryptococcus are conditional pathogenic fungi that commonly infect immunocompromised patients. Over the past few decades, the increase in antifungal resistance has prompted the development of new antifungal agents. In this study, we explored the potential antifungal effects of secretions from Serratia marcescens on Candida spp. and Cryptococcus neoformans. We confirmed that the supernatant of S. marcescens inhibited fungal growth, suppressed hyphal and biofilm formation, and downregulated the expression of hyphae-specific genes and virulence-related genes in Candida spp. and C. neoformans. Furthermore, the S. marcescens supernatant retained biological stability after heat, pH, and protease K treatment. The chemical profile of the S. marcescens supernatant was characterized by ultra-high-performance liquid chromatography–linear ion trap/orbitrap high resolution mass spectrometry analysis and a total of 61 compounds with an mzCloud best match of greater than 70 were identified. In vivo, treatment with the S. marcescens supernatant reduced the mortality of fungi-infected Galleria mellonella. Taken together, our results revealed that the stable antifungal substances in the supernatant of S. marcescens have promising potential applications in the development of new antifungal agents. |