| Title | Prevalence of Indigenous Antibiotic‑Resistant Salmonella Isolates and Their Application to Explore a Lytic Phage vB_SalS_KFSSM with an Intra‑Broad Specificity |
|---|---|
| Author | Jaein Choe1†, Su‑Hyeon Kim1†, Ji Min Han1, Jong‑Hoon Kim2, Mi‑Sun Kwak2, Do‑Won Jeong3, and Mi‑Kyung Park1* |
| Address | 1School of Food Science and Biotechnology, and Food and Bio‑Industry Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea, 2KookminBio Corporation, Seoul 02826, Republic of Korea, 3Department of Food and Nutrition, Dongduk Women’s University, Seoul 02748, Republic of Korea |
| Bibliography | Journal of Microbiology, 61(12),1063-1073, 2023, |
| DOI | 10.1007/s12275-023-00098-6 |
| Key Words | Indigenous Salmonella · Antibiotic-resistant · Lytic phage · Intra-broad specificity · Prevalence |
| Abstract | The consumption of fresh produce has led to increase in antibiotic-resistant (AR) Salmonella outbreaks. In this study, indigenous Salmonella was isolated from a total of two hundred-two samples including fresh produce and agricultural environmental samples in Korea. After biochemical confirmation using the Indole, Methyl Red, Voges-Proskauer, Citrate tests, presumable Salmonella isolates were identified by 16S rRNA sequencing. Identified Salmonella isolates were evaluated for antibiotic susceptibility against twenty-two antibiotics. The specificity and the efficiency of plating (EOP) of vB_SalS_KFSSM were evaluated against fifty-three bacterial strains. Twenty-five suspected Salmonella were isolated and confirmed by the positive result for methyl red and citrate, of which ten were identified as Salmonella spp. through 16S rRNA gene sequencing. Eight Salmonella isolates (4.0%, n = 8/202) were resistant to at least one antibiotic, among which five were multi-drug resistant. As a lytic phage against Salmonella spp. CMGS-1, vB_SalS_KFSSM was isolated from cow manure. The phage was observed as a tailed phage belonging to the class Caudoviricetes. It exhibited an intra-broad specificity against four indigenous AR Salmonella isolates, two indigenous Salmonella isolates, and five other Salmonella serotypes with great efficiencies (EOP ≥ 0.75). Thus, this study suggested the potential of vB_SalS_KFSSM to combat indigenous AR Salmonella. |