| Title | Identification of Auxin from Pseudomonas sp. P7014 for the Rapid Growth of Pleurotus eryngii Mycelium | ||
| Author | Young Min Kang1 and Kye Man Cho2* | ||
| Address | 1Korean Institute of Oriental Medicine, 2Gyeongnam National University of Science and Technology | ||
| Bibliography | Korean Journal of Microbiology, 50(1),15-21, 2014 | ||
| DOI | http://dx.doi.org/10.7845/kjm.2014.3076 | ||
| Key Words | Pleurotus eryngii, Pseudomonas sp. P7014, bioassay, growth promotion, indole acetic acid | ||
| Abstract | The promoting effect of Pseudomonas sp. P7014 on the mycelia growth of Pleurotus eryngii was investigated. An ethyl acetate fraction (F5) from the culture supernatant of the bacteria was confirmed to contain the growth promoting compound (GPC). The GPC was identified to be indole acetic acid (IAA) by TLC, HPLC, MS/MS, and NMR analyses. P. eryngii mycelia grew rapidly both on PDA and in PDB after the treatment of GPC. The promoting concentration of GPC was as low as 1.0 nM. Tryptophan, the aminated form of IAA, was confirmed to be the precursor of IAA. These results suggested that bacterial secreted compound was IAA and plays an important role in promoting growth of mushroom mycelia. | ||
| Download PDF | 50(1)_03_p.15-21.pdf | ||